Tracking alloreactive cell division in vivo

Howard K. Song, Hooman Noorchashm, Yen K. Lieu, Susan Rostami, Siri Atma S. Greeley, Clyde F. Barker, Ali Naji

    Research output: Contribution to journalArticle

    14 Scopus citations


    Background. The study of alloimmune responses has been limited by a lack of assays that can track the behavior of alloreactive lymphocytes in vivo. Here we utilize an experimental system that allows the identification and study of alloreactive CD4+ lymphocytes responding to major histocompatibility antigens in vivo. Methods. Responder mouse lymphocytes were labeled with a fluorescein-based dye, adoptively transferred into irradiated allogeneic stimulator mice, and recovered at serial time points for analysis by flow cytometry. Results. Discrete generations of CD4+ responder lymphocytes proliferating specifically in response to allogeneic MHC class II were distinguished by fluorescein intensity. Successive division of alloreactive CD4+ lymphocytes was traced up to six generations after 60 hr. Conclusions. This experimental system provides information on the division kinetics of alloreactive CD4+ cells. Other applications include immunophenotyping of alloreactive lymphocyte subsets. Further study of systems such as this will allow the detailed characterization of how alloimmune responses are initiated and proceed in vivo.

    Original languageEnglish (US)
    Pages (from-to)297-299
    Number of pages3
    Issue number2
    StatePublished - Jul 27 1999

    ASJC Scopus subject areas

    • Transplantation

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