Tracer and electrical coupling of rat suprachiasmatic nucleus neurons

Z. G. Jiang, Y. Q. Yang, C. N. Allen

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Whole-cell recording from single neurons of the suprachiasmatic nucleus with an electrode containing the tracer neurobiotin resulted in the staining of multiple neurons in 30% of the cases. Typically, one neuron was darkly stained with dendritic processes and an axon clearly visible while other neurons were lightly stained. The darkly-stained cells were identified as the recorded neuron and tracer-coupled to one to five lightly stained neurons. The resting membrane potential, input membrane conductance, membrane capacitance, the decay time constant and the maximum H-current amplitude of the recorded neurons with tracer-coupled cells were not significantly different from those of neurons not showing tracer coupling. Stimulation of the preoptic area activated an antidromic action potential or an all-or-none small slow inward current in some neurons when the synaptic transmission was blocked by a calcium-free/Mn2+ solution. The small slow inward current did not 'collide' with an orthodromically activated action spike suggesting that the current represents the signal from an electrotonically-coupled neuron. In addition, the frequency of biphasic field currents from a neighbouring cell firing were increased by depolarization and decreased by hyperpolarization of the recorded cell. These data demonstrate a chemical and electrical low-resistance coupling of suprachiasmatic nucleus neurons, which could be important in synthesizing the suprachiasmatic nucleus circadian rhythm.

Original languageEnglish (US)
Pages (from-to)1059-1066
Number of pages8
JournalNeuroscience
Volume77
Issue number4
DOIs
StatePublished - Jan 1 1997

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Keywords

  • circadian rhythm
  • dye coupling
  • electrical transmission
  • neurobiotin
  • suprachiasmatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

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