Trabecular response to laser trabeculoplasty is mediated by a factor produced after treatment

J. M B Bradley, D. E. Parshley, J. R. Samples, Ted Acott

Research output: Contribution to journalArticle

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Abstract

Purpose. Laser trabeculoplasty (LTP) induces trabecular insert cell division and juxtacanalicular matrix metalloproteinase (MMP) expression. Treatment of 180 degrees produces responses for 360 degrees, thus implicating signal transmission by a soluble factor(s). To test this hypothesis, we evaluated trabecular responses to medium conditioned by LTP-treated human anterior segment explant cultures. Methods. Human anterior segment explants were given sham or clinical-parameter LTP treatments and allowed to condition culture medium for 8 hrs. This conditioned medium was then applied to fresh explants or trabecular cell cultures. 3H-thymidine incorporation into DNA or MMP protein or mRNA expression was then evaluated. Results. Trabecular insert cell division in explants is increased by exposure to 8-hr conditioned medium from LTP- but not sham-treated explants. Gelatinase B and stromelysin expression by trabecular cells exposed to LTP- but not sham-treatment medium were increased at 24-72 hrs. Alternative types of trabecular wounding did not mimic LTP-treatment. Hypotonic shock and sonication of trabecular meshworks did not release this LTP factor. Conclusion. The trabecular response to LTP involves the production rather than release of a factor(s), which can mediate both the trabecular insert cell division response and the MMP expression response by trabecular cells. This factor may have the potential to replace LTP-treatment as a therapy for glaucoma.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

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Trabeculectomy
Lasers
Conditioned Culture Medium
Matrix Metalloproteinases
Cell Division
Therapeutics
Trabecular Meshwork
Matrix Metalloproteinase 3
Sonication
Osmotic Pressure
Matrix Metalloproteinase 9
Glaucoma
Thymidine
Culture Media
Cell Culture Techniques
Placebos
Messenger RNA
DNA

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Trabecular response to laser trabeculoplasty is mediated by a factor produced after treatment. / Bradley, J. M B; Parshley, D. E.; Samples, J. R.; Acott, Ted.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

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abstract = "Purpose. Laser trabeculoplasty (LTP) induces trabecular insert cell division and juxtacanalicular matrix metalloproteinase (MMP) expression. Treatment of 180 degrees produces responses for 360 degrees, thus implicating signal transmission by a soluble factor(s). To test this hypothesis, we evaluated trabecular responses to medium conditioned by LTP-treated human anterior segment explant cultures. Methods. Human anterior segment explants were given sham or clinical-parameter LTP treatments and allowed to condition culture medium for 8 hrs. This conditioned medium was then applied to fresh explants or trabecular cell cultures. 3H-thymidine incorporation into DNA or MMP protein or mRNA expression was then evaluated. Results. Trabecular insert cell division in explants is increased by exposure to 8-hr conditioned medium from LTP- but not sham-treated explants. Gelatinase B and stromelysin expression by trabecular cells exposed to LTP- but not sham-treatment medium were increased at 24-72 hrs. Alternative types of trabecular wounding did not mimic LTP-treatment. Hypotonic shock and sonication of trabecular meshworks did not release this LTP factor. Conclusion. The trabecular response to LTP involves the production rather than release of a factor(s), which can mediate both the trabecular insert cell division response and the MMP expression response by trabecular cells. This factor may have the potential to replace LTP-treatment as a therapy for glaucoma.",
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