Trabecular meshwork glycosaminoglycans in human and cynomolgus monkey eye

Ted Acott, M. Westcott, M. S. Passo, E. M. Van Buskirk

Research output: Contribution to journalArticle

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Abstract

The glycosaminoglycans (GAGs) extractable from the trabecular meshwork (TM) of humans and non-human primate eye have been analyzed by sequential enzymatic degradation and cellulose acetate electrophoresis. For comparison, similar extracts of the cornea, sclera, iris, and ciliary body have also been analyzed. The distribution of glycosaminoglycans in human and in cynomolgus monkey TMs are similar, although not identical. The human TM contains hyaluronic acid (HA), chondroitin-4-sulfate and/or 6-sulfate (CS), dermatan sulfate (DS), keratan sulfate (KS), heparan sulfate (HS), and an unidentified band of Alcian Blue staining material, which is resistant to the enzymes that we used. Based upon quantitation of the Alcian Blue staining intensities of extracted GAGs, which have been corrected by a relative dye-binding factor, the GAgs of the human TM include: 29.0% HA, 14.1% CS, 21.5% DS, 20.3% KS, and 15.0% HS. The cynomolgus monkey trabecular GAGs include: 12.8% HA, 14,3% CS, 15.2% DS, 42.1% KS, and 15.6% HS.

Original languageEnglish (US)
Pages (from-to)1320-1329
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume26
Issue number10
StatePublished - 1985

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Trabecular Meshwork
Macaca fascicularis
Keratan Sulfate
Glycosaminoglycans
Dermatan Sulfate
Heparitin Sulfate
Hyaluronic Acid
Alcian Blue
Cellulose Acetate Electrophoresis
Staining and Labeling
Ciliary Body
Sclera
Chondroitin Sulfates
Iris
Cornea
Primates
Sulfates
Coloring Agents
Enzymes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Trabecular meshwork glycosaminoglycans in human and cynomolgus monkey eye. / Acott, Ted; Westcott, M.; Passo, M. S.; Van Buskirk, E. M.

In: Investigative Ophthalmology and Visual Science, Vol. 26, No. 10, 1985, p. 1320-1329.

Research output: Contribution to journalArticle

Acott, T, Westcott, M, Passo, MS & Van Buskirk, EM 1985, 'Trabecular meshwork glycosaminoglycans in human and cynomolgus monkey eye', Investigative Ophthalmology and Visual Science, vol. 26, no. 10, pp. 1320-1329.
Acott, Ted ; Westcott, M. ; Passo, M. S. ; Van Buskirk, E. M. / Trabecular meshwork glycosaminoglycans in human and cynomolgus monkey eye. In: Investigative Ophthalmology and Visual Science. 1985 ; Vol. 26, No. 10. pp. 1320-1329.
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