Toxicity of neurons treated with herbicides and neuroprotection by mitochondria-targeted antioxidant SS31

Tejaswini P. Reddy, Maria Manczak, Marcus J. Calkins, Peizhong Mao, Arubala Reddy, Ulziibat Shirendeb, Byung Park, P (Hemachandra) Reddy

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The purpose of this study was to determine the neurotoxicity of two commonly used herbicides: picloram and triclopyr and the neuroprotective effects of the mitochondria-targeted antioxidant, SS31. Using mouse neuroblastoma (N2a) cells and primary neurons from C57BL/6 mice, we investigated the toxicity of these herbicides, and protective effects of SS1 peptide against picloram and triclopyr toxicity. We measured total RNA content, cell viability and mRNA expression of peroxiredoxins, neuroprotective genes, mitochondrial- encoded electron transport chain (ETC) genes in N2a cells treated with herbicides and SS31. Using primary neurons from C57BL/6 mice, neuronal survival was studied in neurons treated with herbicides, in neurons pretreated with SS31 plus treated with herbicides, neurons treated with SS31 alone, and untreated neurons. Significantly decreased total RNA content, and cell viability in N2a cells treated with picloram and triclopyr were found compared to untreated N2a cells. Decreased mRNA expression of neuroprotective genes, and ETC genes in cells treated with herbicides was found compared to untreated cells. Decreased mRNA expression of peroxiredoxins 1-6 in N2a cells treated with picloram was found, suggesting that picloram affects the antioxidant enzymes in N2a cells. Immunofluorescence analysis of primary neurons revealed that decreased neuronal branching and degenerating neurons in neurons treated with picloram and triclopyr. However, neurons pretreated with SS31 prevented degenerative process caused by herbicides. Based on these results, we propose that herbicides-picloram and triclopyr appear to damage neurons, and the SS31 peptide appears to protect neurons from herbicide toxicity.

Original languageEnglish (US)
Pages (from-to)203-221
Number of pages19
JournalInternational Journal of Environmental Research and Public Health
Volume8
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Herbicides
Picloram
Mitochondria
Antioxidants
Neurons
Peroxiredoxins
Electron Transport
Inbred C57BL Mouse
Messenger RNA
Neuroprotection
Cell Survival
RNA
Mitochondrial Genes
Neuroprotective Agents
Neuroblastoma
Genes
Fluorescent Antibody Technique
triclopyr
Gene Expression

Keywords

  • Electron transport chain
  • Herbicides
  • Mitochondria-targeted antioxidant
  • Mouse neuroblastoma cells
  • Mouse primary hippocampal neurons
  • Oxidative stress
  • Picloram
  • Szeto-schiller peptide 31
  • Triclopyr

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Toxicity of neurons treated with herbicides and neuroprotection by mitochondria-targeted antioxidant SS31. / Reddy, Tejaswini P.; Manczak, Maria; Calkins, Marcus J.; Mao, Peizhong; Reddy, Arubala; Shirendeb, Ulziibat; Park, Byung; Reddy, P (Hemachandra).

In: International Journal of Environmental Research and Public Health, Vol. 8, No. 1, 01.2011, p. 203-221.

Research output: Contribution to journalArticle

Reddy, Tejaswini P. ; Manczak, Maria ; Calkins, Marcus J. ; Mao, Peizhong ; Reddy, Arubala ; Shirendeb, Ulziibat ; Park, Byung ; Reddy, P (Hemachandra). / Toxicity of neurons treated with herbicides and neuroprotection by mitochondria-targeted antioxidant SS31. In: International Journal of Environmental Research and Public Health. 2011 ; Vol. 8, No. 1. pp. 203-221.
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