Toxicity of Cr(III) to Shewanella sp. strain MR-4 during Cr(VI) reduction

Rizlan Bencheikh-Latmani, Anna Obraztsova, Mason R. Mackey, Mark H. Ellisman, Bradley Tebo

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Abstract

Bioremediation of chromium through the reduction of hexavalent chromium (as the chromate ion, CrO4 2-) is based on the notion that the product, trivalent chromium (Cr(III)), is less toxic than chromate. In this study, we show that soluble Cr(III), present at pH 6-8 as the Cr3+ ion and/or hydroxyl complexes (henceforth referred to as uncomplexed Cr(III)), can be found transiently in significant concentrations and has a deleterious effect on Shewanella sp. MR-4. However, Cr(III) complexed to an organic ligand or precipitated as Cr(OH)3(s) has little or no effect on cells. Similarly, during the reduction of Cr(VI) by strain MR-4, complexation of the product, Cr(III), results in increased cell survival and extended Cr(VI) reduction activity. These results and gene expression data obtained by qRT-PCR (quantitative reverse transcription-PCR) suggest that the observed toxic effect of Cr(III) formed during Cr(VI) reduction or added as an uncomplexed species is due to the interference with basic cell activities such as DNA transcription and/or replication. Important implications for the bioremediation of Cr(VI)-contaminated sites emerge from this study: Cr(VI) reduction by Shewanella sp. MR-4 is enhanced and sustained by the presence of compounds able to complex Cr(III) as it is being formed but, in turn, the complexation of Cr(III) precludes its precipitation and immobilization.

Original languageEnglish (US)
Pages (from-to)214-220
Number of pages7
JournalEnvironmental Science and Technology
Volume41
Issue number1
DOIs
StatePublished - Jan 1 2007

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Toxicity
toxicity
chromium
Chromium
Chromates
chromate
Bioremediation
Transcription
Complexation
complexation
bioremediation
Poisons
Ions
ion
Gene expression
immobilization
ligand
gene expression
DNA
Hydroxyl Radical

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

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Toxicity of Cr(III) to Shewanella sp. strain MR-4 during Cr(VI) reduction. / Bencheikh-Latmani, Rizlan; Obraztsova, Anna; Mackey, Mason R.; Ellisman, Mark H.; Tebo, Bradley.

In: Environmental Science and Technology, Vol. 41, No. 1, 01.01.2007, p. 214-220.

Research output: Contribution to journalArticle

Bencheikh-Latmani, R, Obraztsova, A, Mackey, MR, Ellisman, MH & Tebo, B 2007, 'Toxicity of Cr(III) to Shewanella sp. strain MR-4 during Cr(VI) reduction', Environmental Science and Technology, vol. 41, no. 1, pp. 214-220. https://doi.org/10.1021/es0622655
Bencheikh-Latmani, Rizlan ; Obraztsova, Anna ; Mackey, Mason R. ; Ellisman, Mark H. ; Tebo, Bradley. / Toxicity of Cr(III) to Shewanella sp. strain MR-4 during Cr(VI) reduction. In: Environmental Science and Technology. 2007 ; Vol. 41, No. 1. pp. 214-220.
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