Toward development of bioactive, neurotransmitter-immobilized surfaces for interaction with post-synaptic membrane receptors

Tothu (Tania) Vu, U. Saifuddin, M. Rezac, H. Qian, D. R. Pepperberg, T. A. Desai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we report progress toward the development of bioactive surfaces based on GABA, a major neurotransmitter in the nervous system. While immobilization techniques have focused largely on antibodies, enzymes, and receptors, to our knowledge, this is the first report of a prototype neurotransmitter-immobilized surface. Biosurfaces were assembled onto either mica or glass using passive adsorption of avidin and subsequent attachment of a derivatized form of GABA via a biotin-avidin affinity bond. Surface characterization was carried out using atomic force microscopy to visualize surface topology. The data reveal (1) passive adsorption of avidin is uniformly dispersed and cluster densities can be controlled through the concentration of the avidin incubation solution (2) GABA tethered via biotin to these avidin surfaces showed a homogenous distribution of the tethered neurotransmitter molecule onto glass and two different population distributions onto mica. Functional assays performed to test the biological activity of the synthesized GABA, direct sandwich ELISA, as well as comparison of AFM images of anti-GABA antibody incubation of these prepared GABA surfaces to control studies using anti-IgG antibody, together support the idea that these synthesized surfaces are biofunctional.

Original languageEnglish (US)
Title of host publication2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages46-50
Number of pages5
ISBN (Print)0780374800, 9780780374805
DOIs
StatePublished - 2002
Externally publishedYes
Event2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States
Duration: May 2 2002May 4 2002

Other

Other2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
CountryUnited States
CityMadison
Period5/2/025/4/02

Keywords

  • AFM
  • biosurface
  • immobilization
  • ligand
  • neurotransmitter
  • solid substrate

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Toward development of bioactive, neurotransmitter-immobilized surfaces for interaction with post-synaptic membrane receptors'. Together they form a unique fingerprint.

  • Cite this

    Vu, T. T., Saifuddin, U., Rezac, M., Qian, H., Pepperberg, D. R., & Desai, T. A. (2002). Toward development of bioactive, neurotransmitter-immobilized surfaces for interaction with post-synaptic membrane receptors. In 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings (pp. 46-50). [1002262] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MMB.2002.1002262