Topical dual-stain difference imaging for rapid intra-operative tumor identification in fresh specimens

Scott C. Davis, Summer L. Gibbs, Jason R. Gunn, Brian W. Pogue

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Assessing tumor margin status during surgery is critical to ensure complete resection of cancer tissue; however, current approaches are ineffective and often result in repeat surgery. We present an optical imaging approach for margin assessment using topical application of two fluorescent stains, one targeted to a tumor biomarker and the other a nontargeted reference, to freshly excised specimens. Computing a normalized difference image from fluorescence images of the targeted and untargeted stains suppresses the confounding effects of nonspecific uptake. Applying this approach in excised breast tumor models produced promising tumor-to-normal tissue contrasts that were significantly higher than single-targeted-stain imaging.

Original languageEnglish (US)
Pages (from-to)5184-5187
Number of pages4
JournalOptics Letters
Volume38
Issue number23
DOIs
StatePublished - Dec 1 2013

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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