Top-down proteomics reveals a unique protein S-thiolation switch in Salmonella Typhimurium in response to infection-like conditions

Charles Ansong, Si Wu, Da Meng, Xiaowen Liu, Heather M. Brewer, Brooke L Deatherage Kaiser, Ernesto S. Nakayasu, John R. Cort, Pavel Pevzner, Richard D. Smith, Fred Heffron, Joshua N. Adkins, Ljiljana Paša-Tolić

    Research output: Contribution to journalArticle

    101 Citations (Scopus)

    Abstract

    Characterization of the mature protein complement in cells is crucial for a better understanding of cellular processes on a systemswide scale. Toward this end, we used single-dimension ultra-highpressure liquid chromatography mass spectrometry to investigate the comprehensive "intact" proteome of the Gram-negative bacterial pathogen Salmonella Typhimurium. Top-down proteomics analysis revealed 563 unique proteins including 1,665 proteoforms generated by posttranslational modifications (PTMs), representing the largest microbial top-down dataset reported to date. We confirmed many previously recognized aspects of Salmonella biology and bacterial PTMs, and our analysis also revealed several additional biological insights. Of particular interest was differential utilization of the protein S-thiolation forms S-glutathionylation and S-cysteinylation in response to infection-like conditions versus basal conditions. This finding of a S-glutathionylation-to-S-cysteinylation switch in a condition-specific manner was corroborated by bottom-up proteomics data and further by changes in corresponding biosynthetic pathways under infection-like conditions and during actual infection of host cells. This differential utilization highlights underlying metabolic mechanisms that modulate changes in cellular signaling, and represents a report of S-cysteinylation in Gram-negative bacteria. Additionally, the functional relevance of these PTMs was supported by protein structure and gene deletion analyses. The demonstrated utility of our simple proteome-wide intact protein level measurement strategy for gaining biological insight should promote broader adoption and applications of top-down proteomics approaches.

    Original languageEnglish (US)
    Pages (from-to)10153-10158
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume110
    Issue number25
    DOIs
    StatePublished - Jun 18 2013

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    Protein S
    Salmonella typhimurium
    Post Translational Protein Processing
    Proteomics
    Proteome
    Infection
    Proteins
    Biosynthetic Pathways
    Gene Deletion
    Gram-Negative Bacteria
    Salmonella
    Liquid Chromatography
    Mass Spectrometry
    Complement System Proteins

    ASJC Scopus subject areas

    • General

    Cite this

    Top-down proteomics reveals a unique protein S-thiolation switch in Salmonella Typhimurium in response to infection-like conditions. / Ansong, Charles; Wu, Si; Meng, Da; Liu, Xiaowen; Brewer, Heather M.; Kaiser, Brooke L Deatherage; Nakayasu, Ernesto S.; Cort, John R.; Pevzner, Pavel; Smith, Richard D.; Heffron, Fred; Adkins, Joshua N.; Paša-Tolić, Ljiljana.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 25, 18.06.2013, p. 10153-10158.

    Research output: Contribution to journalArticle

    Ansong, C, Wu, S, Meng, D, Liu, X, Brewer, HM, Kaiser, BLD, Nakayasu, ES, Cort, JR, Pevzner, P, Smith, RD, Heffron, F, Adkins, JN & Paša-Tolić, L 2013, 'Top-down proteomics reveals a unique protein S-thiolation switch in Salmonella Typhimurium in response to infection-like conditions', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 25, pp. 10153-10158. https://doi.org/10.1073/pnas.1221210110
    Ansong, Charles ; Wu, Si ; Meng, Da ; Liu, Xiaowen ; Brewer, Heather M. ; Kaiser, Brooke L Deatherage ; Nakayasu, Ernesto S. ; Cort, John R. ; Pevzner, Pavel ; Smith, Richard D. ; Heffron, Fred ; Adkins, Joshua N. ; Paša-Tolić, Ljiljana. / Top-down proteomics reveals a unique protein S-thiolation switch in Salmonella Typhimurium in response to infection-like conditions. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 25. pp. 10153-10158.
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    AU - Liu, Xiaowen

    AU - Brewer, Heather M.

    AU - Kaiser, Brooke L Deatherage

    AU - Nakayasu, Ernesto S.

    AU - Cort, John R.

    AU - Pevzner, Pavel

    AU - Smith, Richard D.

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