TNAP activity is localized at critical sites of retinal neurotransmission across various vertebrate species

Orsolya Kántor, Alexandra Varga, Tamás Kovács-Öller, Anna Énzsöly, Lajos Balogh, Gábor Baksa, Zsuzsanna Szepessy, Caroline Fonta, Anna Roe, Roland Nitschke, Ágoston Szél, László Négyessy, Béla Völgyi, Ákos Lukáts

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Evidence is emerging with regard to the role of tissue non-specific alkaline phosphatase (TNAP) in neural functions. As an ectophosphatase, this enzyme might influence neural activity and synaptic transmission in diverse ways. The localization of the enzyme in known neural circuits, such as the retina, might significantly advance an understanding of its role in normal and pathological functioning. However, the presence of TNAP in the retina is scarcely investigated. Our multispecies comparative study (zebrafish, cichlid, frog, chicken, mouse, rat, golden hamster, guinea pig, rabbit, sheep, cat, dog, ferret, squirrel monkey, human) using enzyme histochemistry and Western blots has shown the presence of TNAP activity in the retina of several mammalian species, including humans. Although the TNAP activity pattern varies across species, we have observed the following trends: (1) in all investigated species (except golden hamster), retinal vessels display TNAP activity; (2) TNAP activity consistently occurs in the photoreceptor layer; (3) in majority of the investigated species, marked TNAP activity is present in the outer and inner plexiform layers. In zebrafish, frog, chicken, guinea pig, and rat, TNAP histochemistry has revealed several sublayers of the inner plexiform layer. Frog, golden hamster, guinea pig, mouse, and human retinas possess a subpopulation of amacrine cells positively staining for TNAP activity. The expression of TNAP in critical sites of retinal signal transmission across a wide range of species suggests its fundamental, evolutionally conserved role in vision.

Original languageEnglish (US)
Pages (from-to)85-98
Number of pages14
JournalCell and Tissue Research
Volume358
Issue number1
DOIs
StatePublished - Oct 7 2014
Externally publishedYes

Fingerprint

Synaptic Transmission
Alkaline Phosphatase
Vertebrates
Retina
Mesocricetus
Anura
Guinea Pigs
Zebrafish
Chickens
Enzymes
Amacrine Cells
Cichlids
Saimiri
Retinal Vessels
Ferrets
Sheep
Cats
Western Blotting
Dogs
Staining and Labeling

Keywords

  • Amacrine cells
  • Inner plexiform layer
  • Photoreceptors
  • Retina
  • TNAP

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology
  • Medicine(all)

Cite this

Kántor, O., Varga, A., Kovács-Öller, T., Énzsöly, A., Balogh, L., Baksa, G., ... Lukáts, Á. (2014). TNAP activity is localized at critical sites of retinal neurotransmission across various vertebrate species. Cell and Tissue Research, 358(1), 85-98. https://doi.org/10.1007/s00441-014-1944-3

TNAP activity is localized at critical sites of retinal neurotransmission across various vertebrate species. / Kántor, Orsolya; Varga, Alexandra; Kovács-Öller, Tamás; Énzsöly, Anna; Balogh, Lajos; Baksa, Gábor; Szepessy, Zsuzsanna; Fonta, Caroline; Roe, Anna; Nitschke, Roland; Szél, Ágoston; Négyessy, László; Völgyi, Béla; Lukáts, Ákos.

In: Cell and Tissue Research, Vol. 358, No. 1, 07.10.2014, p. 85-98.

Research output: Contribution to journalArticle

Kántor, O, Varga, A, Kovács-Öller, T, Énzsöly, A, Balogh, L, Baksa, G, Szepessy, Z, Fonta, C, Roe, A, Nitschke, R, Szél, Á, Négyessy, L, Völgyi, B & Lukáts, Á 2014, 'TNAP activity is localized at critical sites of retinal neurotransmission across various vertebrate species', Cell and Tissue Research, vol. 358, no. 1, pp. 85-98. https://doi.org/10.1007/s00441-014-1944-3
Kántor, Orsolya ; Varga, Alexandra ; Kovács-Öller, Tamás ; Énzsöly, Anna ; Balogh, Lajos ; Baksa, Gábor ; Szepessy, Zsuzsanna ; Fonta, Caroline ; Roe, Anna ; Nitschke, Roland ; Szél, Ágoston ; Négyessy, László ; Völgyi, Béla ; Lukáts, Ákos. / TNAP activity is localized at critical sites of retinal neurotransmission across various vertebrate species. In: Cell and Tissue Research. 2014 ; Vol. 358, No. 1. pp. 85-98.
@article{c3ec921fd42c4a24862804b25503a589,
title = "TNAP activity is localized at critical sites of retinal neurotransmission across various vertebrate species",
abstract = "Evidence is emerging with regard to the role of tissue non-specific alkaline phosphatase (TNAP) in neural functions. As an ectophosphatase, this enzyme might influence neural activity and synaptic transmission in diverse ways. The localization of the enzyme in known neural circuits, such as the retina, might significantly advance an understanding of its role in normal and pathological functioning. However, the presence of TNAP in the retina is scarcely investigated. Our multispecies comparative study (zebrafish, cichlid, frog, chicken, mouse, rat, golden hamster, guinea pig, rabbit, sheep, cat, dog, ferret, squirrel monkey, human) using enzyme histochemistry and Western blots has shown the presence of TNAP activity in the retina of several mammalian species, including humans. Although the TNAP activity pattern varies across species, we have observed the following trends: (1) in all investigated species (except golden hamster), retinal vessels display TNAP activity; (2) TNAP activity consistently occurs in the photoreceptor layer; (3) in majority of the investigated species, marked TNAP activity is present in the outer and inner plexiform layers. In zebrafish, frog, chicken, guinea pig, and rat, TNAP histochemistry has revealed several sublayers of the inner plexiform layer. Frog, golden hamster, guinea pig, mouse, and human retinas possess a subpopulation of amacrine cells positively staining for TNAP activity. The expression of TNAP in critical sites of retinal signal transmission across a wide range of species suggests its fundamental, evolutionally conserved role in vision.",
keywords = "Amacrine cells, Inner plexiform layer, Photoreceptors, Retina, TNAP",
author = "Orsolya K{\'a}ntor and Alexandra Varga and Tam{\'a}s Kov{\'a}cs-{\"O}ller and Anna {\'E}nzs{\"o}ly and Lajos Balogh and G{\'a}bor Baksa and Zsuzsanna Szepessy and Caroline Fonta and Anna Roe and Roland Nitschke and {\'A}goston Sz{\'e}l and L{\'a}szl{\'o} N{\'e}gyessy and B{\'e}la V{\"o}lgyi and {\'A}kos Luk{\'a}ts",
year = "2014",
month = "10",
day = "7",
doi = "10.1007/s00441-014-1944-3",
language = "English (US)",
volume = "358",
pages = "85--98",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - TNAP activity is localized at critical sites of retinal neurotransmission across various vertebrate species

AU - Kántor, Orsolya

AU - Varga, Alexandra

AU - Kovács-Öller, Tamás

AU - Énzsöly, Anna

AU - Balogh, Lajos

AU - Baksa, Gábor

AU - Szepessy, Zsuzsanna

AU - Fonta, Caroline

AU - Roe, Anna

AU - Nitschke, Roland

AU - Szél, Ágoston

AU - Négyessy, László

AU - Völgyi, Béla

AU - Lukáts, Ákos

PY - 2014/10/7

Y1 - 2014/10/7

N2 - Evidence is emerging with regard to the role of tissue non-specific alkaline phosphatase (TNAP) in neural functions. As an ectophosphatase, this enzyme might influence neural activity and synaptic transmission in diverse ways. The localization of the enzyme in known neural circuits, such as the retina, might significantly advance an understanding of its role in normal and pathological functioning. However, the presence of TNAP in the retina is scarcely investigated. Our multispecies comparative study (zebrafish, cichlid, frog, chicken, mouse, rat, golden hamster, guinea pig, rabbit, sheep, cat, dog, ferret, squirrel monkey, human) using enzyme histochemistry and Western blots has shown the presence of TNAP activity in the retina of several mammalian species, including humans. Although the TNAP activity pattern varies across species, we have observed the following trends: (1) in all investigated species (except golden hamster), retinal vessels display TNAP activity; (2) TNAP activity consistently occurs in the photoreceptor layer; (3) in majority of the investigated species, marked TNAP activity is present in the outer and inner plexiform layers. In zebrafish, frog, chicken, guinea pig, and rat, TNAP histochemistry has revealed several sublayers of the inner plexiform layer. Frog, golden hamster, guinea pig, mouse, and human retinas possess a subpopulation of amacrine cells positively staining for TNAP activity. The expression of TNAP in critical sites of retinal signal transmission across a wide range of species suggests its fundamental, evolutionally conserved role in vision.

AB - Evidence is emerging with regard to the role of tissue non-specific alkaline phosphatase (TNAP) in neural functions. As an ectophosphatase, this enzyme might influence neural activity and synaptic transmission in diverse ways. The localization of the enzyme in known neural circuits, such as the retina, might significantly advance an understanding of its role in normal and pathological functioning. However, the presence of TNAP in the retina is scarcely investigated. Our multispecies comparative study (zebrafish, cichlid, frog, chicken, mouse, rat, golden hamster, guinea pig, rabbit, sheep, cat, dog, ferret, squirrel monkey, human) using enzyme histochemistry and Western blots has shown the presence of TNAP activity in the retina of several mammalian species, including humans. Although the TNAP activity pattern varies across species, we have observed the following trends: (1) in all investigated species (except golden hamster), retinal vessels display TNAP activity; (2) TNAP activity consistently occurs in the photoreceptor layer; (3) in majority of the investigated species, marked TNAP activity is present in the outer and inner plexiform layers. In zebrafish, frog, chicken, guinea pig, and rat, TNAP histochemistry has revealed several sublayers of the inner plexiform layer. Frog, golden hamster, guinea pig, mouse, and human retinas possess a subpopulation of amacrine cells positively staining for TNAP activity. The expression of TNAP in critical sites of retinal signal transmission across a wide range of species suggests its fundamental, evolutionally conserved role in vision.

KW - Amacrine cells

KW - Inner plexiform layer

KW - Photoreceptors

KW - Retina

KW - TNAP

UR - http://www.scopus.com/inward/record.url?scp=84919864956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84919864956&partnerID=8YFLogxK

U2 - 10.1007/s00441-014-1944-3

DO - 10.1007/s00441-014-1944-3

M3 - Article

VL - 358

SP - 85

EP - 98

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 1

ER -