TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects

Min Kyung Choo, Yasuyo Sano, Changhoon Kim, Kei Yasuda, Xiao Dong Li, Xin Lin, Mary Stenzel-Poore, Lena Alexopoulou, Sankar Ghosh, Eicke Latz, Ian R. Rifkin, Zhijian J. Chen, George C. Stewart, Hyonyong Chong, Jin Mo Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The spores of pathogenic bacteria are involved in host entry and the initial encounter with the host immune system. How bacterial spores interact with host immunity, however, remains poorly understood. Here, we show that the spores of Bacillus anthracis (BA), the etiologic agent of anthrax, possess an intrinsic ability to induce host immune responses. This immunostimulatory activity is attributable to high amounts of RNA present in the spore surface layer. RNA-sensing TLRs, TLR7, and TLR13 in mice and their human counterparts, are responsible for detecting and triggering the host cell response to BA spores, whereas TLR2 mediates the sensing of vegetative BA. BA spores, but not vegetative BA, induce type I IFN (IFN-I) production. Although TLR signaling in itself affords protection against BA, spore RNA-induced IFN-I signaling is disruptive to BA clearance. Our study suggests a role for bacterial spore-associated RNA in microbial pathogenesis and illustrates a little known aspect of interactions between the host and spore-forming bacteria.

Original languageEnglish (US)
Pages (from-to)1297-1311
Number of pages15
JournalJournal of Experimental Medicine
Volume214
Issue number5
DOIs
StatePublished - May 1 2017

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Bacterial Spores
Bacillus anthracis
Spores
RNA
Bacteria
Anthrax
Immune System
Immunity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects. / Choo, Min Kyung; Sano, Yasuyo; Kim, Changhoon; Yasuda, Kei; Li, Xiao Dong; Lin, Xin; Stenzel-Poore, Mary; Alexopoulou, Lena; Ghosh, Sankar; Latz, Eicke; Rifkin, Ian R.; Chen, Zhijian J.; Stewart, George C.; Chong, Hyonyong; Park, Jin Mo.

In: Journal of Experimental Medicine, Vol. 214, No. 5, 01.05.2017, p. 1297-1311.

Research output: Contribution to journalArticle

Choo, MK, Sano, Y, Kim, C, Yasuda, K, Li, XD, Lin, X, Stenzel-Poore, M, Alexopoulou, L, Ghosh, S, Latz, E, Rifkin, IR, Chen, ZJ, Stewart, GC, Chong, H & Park, JM 2017, 'TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects', Journal of Experimental Medicine, vol. 214, no. 5, pp. 1297-1311. https://doi.org/10.1084/jem.20161141
Choo, Min Kyung ; Sano, Yasuyo ; Kim, Changhoon ; Yasuda, Kei ; Li, Xiao Dong ; Lin, Xin ; Stenzel-Poore, Mary ; Alexopoulou, Lena ; Ghosh, Sankar ; Latz, Eicke ; Rifkin, Ian R. ; Chen, Zhijian J. ; Stewart, George C. ; Chong, Hyonyong ; Park, Jin Mo. / TLR sensing of bacterial spore-associated RNA triggers host immune responses with detrimental effects. In: Journal of Experimental Medicine. 2017 ; Vol. 214, No. 5. pp. 1297-1311.
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