Titration and neutralizing antibody quantification by focus forming assay for Powassan virus

E. Taylor Stone; Alec J. Hirsch; Jessica L. Smith; James D. Brien; Amelia K. Pinto

doi:10.1016/j.xpro.2022.101473

Titration and neutralizing antibody quantification by focus forming assay for Powassan virus

E. Taylor Stone, Alec J. Hirsch, Jessica L. Smith, James D. Brien, Amelia K. Pinto

Vaccine and Gene Therapy Institute

Research output: Contribution to journal › Article › peer-review

Abstract

The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies. By adapting focus-forming assays previously optimized for West Nile virus and Zika virus, this protocol is able to determine viral load, evaluate antivirals, and measure neutralizing antibodies. Although limited by its requirement of a detection antibody, this protocol includes a rapid and high-throughput assay for measuring viral titer. By utilizing a baby hamster kidney cell line and a 96-well plate format, this protocol allows for more sensitivity in the detection of POWV lineage I. For complete details on the use and execution of this protocol, please refer to Stone et al. (2022).

Original language	English (US)
Article number	101473
Journal	STAR Protocols
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2022.101473
State	Published - Sep 16 2022

Keywords

Antibody
Cell-based Assays
Microbiology

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2022.101473

Cite this

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title = "Titration and neutralizing antibody quantification by focus forming assay for Powassan virus",

abstract = "The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies. By adapting focus-forming assays previously optimized for West Nile virus and Zika virus, this protocol is able to determine viral load, evaluate antivirals, and measure neutralizing antibodies. Although limited by its requirement of a detection antibody, this protocol includes a rapid and high-throughput assay for measuring viral titer. By utilizing a baby hamster kidney cell line and a 96-well plate format, this protocol allows for more sensitivity in the detection of POWV lineage I. For complete details on the use and execution of this protocol, please refer to Stone et al. (2022).",

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AU - Stone, E. Taylor

AU - Hirsch, Alec J.

AU - Smith, Jessica L.

AU - Brien, James D.

AU - Pinto, Amelia K.

PY - 2022/9/16

Y1 - 2022/9/16

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AB - The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies. By adapting focus-forming assays previously optimized for West Nile virus and Zika virus, this protocol is able to determine viral load, evaluate antivirals, and measure neutralizing antibodies. Although limited by its requirement of a detection antibody, this protocol includes a rapid and high-throughput assay for measuring viral titer. By utilizing a baby hamster kidney cell line and a 96-well plate format, this protocol allows for more sensitivity in the detection of POWV lineage I. For complete details on the use and execution of this protocol, please refer to Stone et al. (2022).

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Titration and neutralizing antibody quantification by focus forming assay for Powassan virus

Abstract

Keywords

ASJC Scopus subject areas

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