Tissue-specific posttranslational processing of pre-prosomatostatin encoded by a metallothionein-somatostatin fusion gene in transgenic mice

Malcolm J. Low, Robert E. Hammer, Richard Goodman, Joel F. Habener, Richard D. Palmiter, Ralph L. Brinster

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The somatostatins are neuropeptides of 14 and 28 amino acids that inhibit the release of growth hormone and other hypophyseal and gastrointestinal peptides. These neuropeptides are cleaved posttranslationally from a common precursor, pre-prosomatostatin. We report here the production and processing of pre-prosomatostatin by transgenic mice carrying a metallothionein-somatostatin fusion gene. The most active site of somatostatin production, as determined by hormone concentrations in the tissues, is the anterior pituitary, a tissue that does not normally synthesize somatostatin-like peptides. Anterior pituitary processed pre-prosomatostatin almost exclusively to the two biologically active peptides, somatostatin-14 and somatostatin-28, whereas the liver and kidney synthesized much smaller quantities of predominantly a 6000 dalton somatostatin-like peptide. The growth of the transgenic mice was normal despite high plasma levels of the somatostatin-like peptides. These studies indicate that proteases which cleave prosomatostatin to somatostatin-28 and somatostatin-14 are not specific to tissues that normally express somatostatin.

Original languageEnglish (US)
Pages (from-to)211-219
Number of pages9
JournalCell
Volume41
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Metallothionein
Gene Fusion
Somatostatin
Transgenic Mice
Fusion reactions
Somatostatin-28
Genes
Tissue
Processing
Neuropeptides
Peptides
Liver
Growth Hormone
prosomatostatin
Catalytic Domain
Peptide Hydrolases
Hormones
Kidney
Plasmas
Amino Acids

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Tissue-specific posttranslational processing of pre-prosomatostatin encoded by a metallothionein-somatostatin fusion gene in transgenic mice. / Low, Malcolm J.; Hammer, Robert E.; Goodman, Richard; Habener, Joel F.; Palmiter, Richard D.; Brinster, Ralph L.

In: Cell, Vol. 41, No. 1, 1985, p. 211-219.

Research output: Contribution to journalArticle

Low, Malcolm J. ; Hammer, Robert E. ; Goodman, Richard ; Habener, Joel F. ; Palmiter, Richard D. ; Brinster, Ralph L. / Tissue-specific posttranslational processing of pre-prosomatostatin encoded by a metallothionein-somatostatin fusion gene in transgenic mice. In: Cell. 1985 ; Vol. 41, No. 1. pp. 211-219.
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