Tissue remodeling in the acute otitis media mouse model

Nathan Sautter, Katherine L. Delaney, Frances A. Hausman, Dennis Trune

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objectives: Otitis media is an infectious, inflammatory process involving the middle ear space. Chronic inflammation is associated with fibrosis, scarring and osteogenesis within the middle ear, which may contribute to subsequent hearing loss and increase the difficulty of treatment. Methods: Heat-killed Streptococcus pneumoniae was injected into the middle ears of 8-12 week old Balb/c mice. Control mice were treated with PBS middle ear injections. Middle ears were harvested at 1, 3, 5 and 7 days following injection (n= 8 for each time point). The middle ears were processed using standard RT-PCR techniques. Up- and down-regulation of mRNA expression of various members of the Bone Morphogenetic Protein (BMP), Fibroblast Growth Factor (FGF) and Matrix Metalloproteinase (MMP) families was quantified and compared to PBS treated controls (n= 8 for each time point). Results: Significant upregulation of MMP2, MMP3 and MMP9 was observed at varying time points (p

Original languageEnglish (US)
Pages (from-to)1368-1371
Number of pages4
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume75
Issue number11
DOIs
StatePublished - Nov 2011

Fingerprint

Otitis Media
Middle Ear
Up-Regulation
Bone Morphogenetic Proteins
Injections
Fibroblast Growth Factors
Streptococcus pneumoniae
Matrix Metalloproteinases
Hearing Loss
Osteogenesis
Cicatrix
Fibrosis
Down-Regulation
Hot Temperature
Inflammation
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Cytokines
  • Mouse model
  • Otitis media
  • Tissue remodeling

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Pediatrics, Perinatology, and Child Health

Cite this

Tissue remodeling in the acute otitis media mouse model. / Sautter, Nathan; Delaney, Katherine L.; Hausman, Frances A.; Trune, Dennis.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 75, No. 11, 11.2011, p. 1368-1371.

Research output: Contribution to journalArticle

Sautter, Nathan ; Delaney, Katherine L. ; Hausman, Frances A. ; Trune, Dennis. / Tissue remodeling in the acute otitis media mouse model. In: International Journal of Pediatric Otorhinolaryngology. 2011 ; Vol. 75, No. 11. pp. 1368-1371.
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