Timing of cardiomyocyte growth, maturation, and attrition in perinatal sheep

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Studies in altricial rodents attribute dramatic changes in perinatal cardiomyocyte growth, maturation, and attrition to stimuli associated with birth. Our purpose was to determine whether birth is a critical trigger controlling perinatal cardiomyocyte growth, maturation and attrition in a precocial large mammal, sheep (Ovis aries). Hearts from0-61 d postnatal lambs were dissected or enzymatically dissociated.Cardiomyocytes weremeasured by micromorphometry, cell cycle activity assessed by immunohistochemistry, and nuclear number counted after DNA staining. Integration of this new data with published fetal data from our laboratory demonstrate that a newly appreciated >30% decrease in myocyte number occurred in the last 10 d of gestation (P <0.0005) concomitant with an increase in cleaved poly (ADP-ribose) polymerase 1 (P <0.05), indicative of apoptosis. Bisegmental linear regressions show that most changes in myocyte growth kinetics occur before birth (median = 15.2 d; P <0.05). Right ventricular but not left ventricular cell number increases in the neonate, by 68% between birth and 60 d postnatal (P = 0.028). We conclude that in sheep few developmental changes in cardiomyocytes result frombirth, excepting the different postnatal degrees of free wall hypertrophy between the ventricles. Furthermore, myocyte number is reduced in both ventricles immediately before term, but proliferation increases myocyte number in the neonatal right ventricle. - Jonker, S. S., Louey, S., Giraud, G. D., Thornburg, K. L., Faber, J. J. Timing of cardiomyocyte growth, maturation, and attrition in perinatal sheep.

    Original languageEnglish (US)
    Pages (from-to)4346-4357
    Number of pages12
    JournalFASEB Journal
    Volume29
    Issue number10
    DOIs
    StatePublished - Oct 1 2015

    Fingerprint

    Cardiac Myocytes
    Sheep
    Muscle Cells
    Parturition
    Growth
    Mammals
    Poly(ADP-ribose) Polymerases
    Growth kinetics
    Linear regression
    Domestic Sheep
    Cells
    Apoptosis
    DNA
    Hypertrophy
    Heart Ventricles
    Rodentia
    Linear Models
    Cell Cycle
    Cell Count
    Immunohistochemistry

    Keywords

    • Apoptosis
    • Cell number
    • Fetus
    • Neonate
    • Proliferation

    ASJC Scopus subject areas

    • Biochemistry
    • Biotechnology
    • Genetics
    • Molecular Biology

    Cite this

    Timing of cardiomyocyte growth, maturation, and attrition in perinatal sheep. / Jonker, Sonnet; Louey, Samantha; Giraud, George; Thornburg, Kent; Faber, J. Job.

    In: FASEB Journal, Vol. 29, No. 10, 01.10.2015, p. 4346-4357.

    Research output: Contribution to journalArticle

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