Time-related changes induced by zinc-deficient diet in the concentration of rat cheek epithelial membrane-coating granules

Nasser Said-Al-Naief, Shahid H. Ashrafi

Research output: Contribution to journalArticle

Abstract

Weanling male Sprague-Dawley rats were fed a diet containing 0.4 parts/ 106 zinc and controls were fed an identical diet supplemented with 40 parts/ 106 zinc. After 9, 18 and 27 days of zinc deficiency, specimens were excised from cheek epithelium and processed for transmission electron microscopy to study the concentration of membrane-coating granules (MCG). Their concentration was increased in the granular-cell layers of the zinc-deficient epithelium and became significantly greater after 18 and 27 days than 9 days of deficiency. MCGs appeared in the parakeratinized layers of zinc-deficient epithelium and their concentration became significantly greater after 27 days in comparison with 9 and 18 days of deficiency. Thus the intracellular retention of MCGs was increased in the granular and parakeratinized layers with the increase in time of zinc deficiency.

Original languageEnglish (US)
Pages (from-to)717-722
Number of pages6
JournalArchives of Oral Biology
Volume40
Issue number8
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Cheek
Zinc
Diet
Membranes
Epithelium
Transmission Electron Microscopy
Sprague Dawley Rats

Keywords

  • granular cells
  • keratinization
  • rat buccal cheek epithelium
  • time-related changes
  • zinc deficiency

ASJC Scopus subject areas

  • Cell Biology
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Time-related changes induced by zinc-deficient diet in the concentration of rat cheek epithelial membrane-coating granules. / Said-Al-Naief, Nasser; Ashrafi, Shahid H.

In: Archives of Oral Biology, Vol. 40, No. 8, 1995, p. 717-722.

Research output: Contribution to journalArticle

@article{1d761fe0ccaf45ee99c623ce4070677e,
title = "Time-related changes induced by zinc-deficient diet in the concentration of rat cheek epithelial membrane-coating granules",
abstract = "Weanling male Sprague-Dawley rats were fed a diet containing 0.4 parts/ 106 zinc and controls were fed an identical diet supplemented with 40 parts/ 106 zinc. After 9, 18 and 27 days of zinc deficiency, specimens were excised from cheek epithelium and processed for transmission electron microscopy to study the concentration of membrane-coating granules (MCG). Their concentration was increased in the granular-cell layers of the zinc-deficient epithelium and became significantly greater after 18 and 27 days than 9 days of deficiency. MCGs appeared in the parakeratinized layers of zinc-deficient epithelium and their concentration became significantly greater after 27 days in comparison with 9 and 18 days of deficiency. Thus the intracellular retention of MCGs was increased in the granular and parakeratinized layers with the increase in time of zinc deficiency.",
keywords = "granular cells, keratinization, rat buccal cheek epithelium, time-related changes, zinc deficiency",
author = "Nasser Said-Al-Naief and Ashrafi, {Shahid H.}",
year = "1995",
doi = "10.1016/0003-9969(95)00034-M",
language = "English (US)",
volume = "40",
pages = "717--722",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",
number = "8",

}

TY - JOUR

T1 - Time-related changes induced by zinc-deficient diet in the concentration of rat cheek epithelial membrane-coating granules

AU - Said-Al-Naief, Nasser

AU - Ashrafi, Shahid H.

PY - 1995

Y1 - 1995

N2 - Weanling male Sprague-Dawley rats were fed a diet containing 0.4 parts/ 106 zinc and controls were fed an identical diet supplemented with 40 parts/ 106 zinc. After 9, 18 and 27 days of zinc deficiency, specimens were excised from cheek epithelium and processed for transmission electron microscopy to study the concentration of membrane-coating granules (MCG). Their concentration was increased in the granular-cell layers of the zinc-deficient epithelium and became significantly greater after 18 and 27 days than 9 days of deficiency. MCGs appeared in the parakeratinized layers of zinc-deficient epithelium and their concentration became significantly greater after 27 days in comparison with 9 and 18 days of deficiency. Thus the intracellular retention of MCGs was increased in the granular and parakeratinized layers with the increase in time of zinc deficiency.

AB - Weanling male Sprague-Dawley rats were fed a diet containing 0.4 parts/ 106 zinc and controls were fed an identical diet supplemented with 40 parts/ 106 zinc. After 9, 18 and 27 days of zinc deficiency, specimens were excised from cheek epithelium and processed for transmission electron microscopy to study the concentration of membrane-coating granules (MCG). Their concentration was increased in the granular-cell layers of the zinc-deficient epithelium and became significantly greater after 18 and 27 days than 9 days of deficiency. MCGs appeared in the parakeratinized layers of zinc-deficient epithelium and their concentration became significantly greater after 27 days in comparison with 9 and 18 days of deficiency. Thus the intracellular retention of MCGs was increased in the granular and parakeratinized layers with the increase in time of zinc deficiency.

KW - granular cells

KW - keratinization

KW - rat buccal cheek epithelium

KW - time-related changes

KW - zinc deficiency

UR - http://www.scopus.com/inward/record.url?scp=0029353143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029353143&partnerID=8YFLogxK

U2 - 10.1016/0003-9969(95)00034-M

DO - 10.1016/0003-9969(95)00034-M

M3 - Article

C2 - 7487572

AN - SCOPUS:0029353143

VL - 40

SP - 717

EP - 722

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 8

ER -