Time course of IL-6 expression in experimental CNS ischemia

Wayne Clark, Lisa G. Rinker, Nikola S. Lessov, Kristin Hazel, Felix Eckenstein

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Interleukin-6 (IL-6) appears to be an important modulator of the inflammatory response associated with CNS ischemia. Clinically, IL-6 values obtained in the first week post-stroke have been shown to correlate with infarct size and outcome. In this study we used a focal reversible stroke model to investigate the time course and relationship to outcome of IL-6 production in plasma, brain and CSF. Reversible middle cerebral artery occlusion or sham surgery was produced in 50 adult Swiss Webster mice by advancing an 8-0 filament into the internal carotid artery for 2 h (sham 1 min). At 3, 6, 12, 24, and 72 h (8 each ischemia; 2 each sham) groups of animals were evaluated on a 28 point clinical scale, blood and CSF obtained, and the brains were evaluated for infarct volume and IL-6 mRNA levels. Serum levels of IL-6 (ELISA mean ± SD; undetectable in controls) overall sham group, 102 ± 87; 3 h, 908 ± 494 * pg ml-1; 6 h, 1079 ± 468 * pg ml- 1; 12 h, 980 ± 221 * pg ml-1; 24 h, 320 ± 314 * pg ml-1; 72 h, 20 ± 30 * pg ml-1 (* p <0.05 to sham). CSF levels (ELISA) overall sham group, 10 ± 18; 3 h, 379 ± 210 * pg ml-1; 6 h, 157 ± 61 * pg ml-1; 12 h, 136 ± 88 * pg ml-1; 24 h, 127 ± 99 pg ml-1; 72 h, 72 ± 9 * pg ml- 1 (* p ≤ 0.05 to sham). Brain IL-6 mRNA levels overall sham group, 20; 3 h, 480; 6 h, 599; 12 h, 7960; 24 h, 20267; 72 h, 0. There was an overall R2 of 0.20 between plasma and CSF IL-6. There was an overall R2 of 0.13 and 0.20 between infarct size and serum and CSF IL-6 level respectively, and an overall R2 of 0.10 and 0.17 between neurologic function and serum and CSF IL-6 level respectively. These findings confirm that IL-6 values increase following CNS ischemia with peak serum and CSF levels occurring before brain values. CSF IL-6 levels had a stronger correlation with neurologic function and infarct size than serum.

Original languageEnglish (US)
Pages (from-to)287-292
Number of pages6
JournalNeurological Research
Volume21
Issue number3
StatePublished - 1999

Fingerprint

Interleukin-6
Ischemia
Serum
Brain
Nervous System
Enzyme-Linked Immunosorbent Assay
Stroke
Messenger RNA
Middle Cerebral Artery Infarction
Internal Carotid Artery

Keywords

  • CNS ischemia
  • Infarct size
  • Inflammatory response
  • Interleukin-6
  • Neurologic function

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Clark, W., Rinker, L. G., Lessov, N. S., Hazel, K., & Eckenstein, F. (1999). Time course of IL-6 expression in experimental CNS ischemia. Neurological Research, 21(3), 287-292.

Time course of IL-6 expression in experimental CNS ischemia. / Clark, Wayne; Rinker, Lisa G.; Lessov, Nikola S.; Hazel, Kristin; Eckenstein, Felix.

In: Neurological Research, Vol. 21, No. 3, 1999, p. 287-292.

Research output: Contribution to journalArticle

Clark, W, Rinker, LG, Lessov, NS, Hazel, K & Eckenstein, F 1999, 'Time course of IL-6 expression in experimental CNS ischemia', Neurological Research, vol. 21, no. 3, pp. 287-292.
Clark W, Rinker LG, Lessov NS, Hazel K, Eckenstein F. Time course of IL-6 expression in experimental CNS ischemia. Neurological Research. 1999;21(3):287-292.
Clark, Wayne ; Rinker, Lisa G. ; Lessov, Nikola S. ; Hazel, Kristin ; Eckenstein, Felix. / Time course of IL-6 expression in experimental CNS ischemia. In: Neurological Research. 1999 ; Vol. 21, No. 3. pp. 287-292.
@article{97062742e0f14d1bbfe0d03f2be40e16,
title = "Time course of IL-6 expression in experimental CNS ischemia",
abstract = "Interleukin-6 (IL-6) appears to be an important modulator of the inflammatory response associated with CNS ischemia. Clinically, IL-6 values obtained in the first week post-stroke have been shown to correlate with infarct size and outcome. In this study we used a focal reversible stroke model to investigate the time course and relationship to outcome of IL-6 production in plasma, brain and CSF. Reversible middle cerebral artery occlusion or sham surgery was produced in 50 adult Swiss Webster mice by advancing an 8-0 filament into the internal carotid artery for 2 h (sham 1 min). At 3, 6, 12, 24, and 72 h (8 each ischemia; 2 each sham) groups of animals were evaluated on a 28 point clinical scale, blood and CSF obtained, and the brains were evaluated for infarct volume and IL-6 mRNA levels. Serum levels of IL-6 (ELISA mean ± SD; undetectable in controls) overall sham group, 102 ± 87; 3 h, 908 ± 494 * pg ml-1; 6 h, 1079 ± 468 * pg ml- 1; 12 h, 980 ± 221 * pg ml-1; 24 h, 320 ± 314 * pg ml-1; 72 h, 20 ± 30 * pg ml-1 (* p <0.05 to sham). CSF levels (ELISA) overall sham group, 10 ± 18; 3 h, 379 ± 210 * pg ml-1; 6 h, 157 ± 61 * pg ml-1; 12 h, 136 ± 88 * pg ml-1; 24 h, 127 ± 99 pg ml-1; 72 h, 72 ± 9 * pg ml- 1 (* p ≤ 0.05 to sham). Brain IL-6 mRNA levels overall sham group, 20; 3 h, 480; 6 h, 599; 12 h, 7960; 24 h, 20267; 72 h, 0. There was an overall R2 of 0.20 between plasma and CSF IL-6. There was an overall R2 of 0.13 and 0.20 between infarct size and serum and CSF IL-6 level respectively, and an overall R2 of 0.10 and 0.17 between neurologic function and serum and CSF IL-6 level respectively. These findings confirm that IL-6 values increase following CNS ischemia with peak serum and CSF levels occurring before brain values. CSF IL-6 levels had a stronger correlation with neurologic function and infarct size than serum.",
keywords = "CNS ischemia, Infarct size, Inflammatory response, Interleukin-6, Neurologic function",
author = "Wayne Clark and Rinker, {Lisa G.} and Lessov, {Nikola S.} and Kristin Hazel and Felix Eckenstein",
year = "1999",
language = "English (US)",
volume = "21",
pages = "287--292",
journal = "Neurological Research",
issn = "0161-6412",
publisher = "Maney Publishing",
number = "3",

}

TY - JOUR

T1 - Time course of IL-6 expression in experimental CNS ischemia

AU - Clark, Wayne

AU - Rinker, Lisa G.

AU - Lessov, Nikola S.

AU - Hazel, Kristin

AU - Eckenstein, Felix

PY - 1999

Y1 - 1999

N2 - Interleukin-6 (IL-6) appears to be an important modulator of the inflammatory response associated with CNS ischemia. Clinically, IL-6 values obtained in the first week post-stroke have been shown to correlate with infarct size and outcome. In this study we used a focal reversible stroke model to investigate the time course and relationship to outcome of IL-6 production in plasma, brain and CSF. Reversible middle cerebral artery occlusion or sham surgery was produced in 50 adult Swiss Webster mice by advancing an 8-0 filament into the internal carotid artery for 2 h (sham 1 min). At 3, 6, 12, 24, and 72 h (8 each ischemia; 2 each sham) groups of animals were evaluated on a 28 point clinical scale, blood and CSF obtained, and the brains were evaluated for infarct volume and IL-6 mRNA levels. Serum levels of IL-6 (ELISA mean ± SD; undetectable in controls) overall sham group, 102 ± 87; 3 h, 908 ± 494 * pg ml-1; 6 h, 1079 ± 468 * pg ml- 1; 12 h, 980 ± 221 * pg ml-1; 24 h, 320 ± 314 * pg ml-1; 72 h, 20 ± 30 * pg ml-1 (* p <0.05 to sham). CSF levels (ELISA) overall sham group, 10 ± 18; 3 h, 379 ± 210 * pg ml-1; 6 h, 157 ± 61 * pg ml-1; 12 h, 136 ± 88 * pg ml-1; 24 h, 127 ± 99 pg ml-1; 72 h, 72 ± 9 * pg ml- 1 (* p ≤ 0.05 to sham). Brain IL-6 mRNA levels overall sham group, 20; 3 h, 480; 6 h, 599; 12 h, 7960; 24 h, 20267; 72 h, 0. There was an overall R2 of 0.20 between plasma and CSF IL-6. There was an overall R2 of 0.13 and 0.20 between infarct size and serum and CSF IL-6 level respectively, and an overall R2 of 0.10 and 0.17 between neurologic function and serum and CSF IL-6 level respectively. These findings confirm that IL-6 values increase following CNS ischemia with peak serum and CSF levels occurring before brain values. CSF IL-6 levels had a stronger correlation with neurologic function and infarct size than serum.

AB - Interleukin-6 (IL-6) appears to be an important modulator of the inflammatory response associated with CNS ischemia. Clinically, IL-6 values obtained in the first week post-stroke have been shown to correlate with infarct size and outcome. In this study we used a focal reversible stroke model to investigate the time course and relationship to outcome of IL-6 production in plasma, brain and CSF. Reversible middle cerebral artery occlusion or sham surgery was produced in 50 adult Swiss Webster mice by advancing an 8-0 filament into the internal carotid artery for 2 h (sham 1 min). At 3, 6, 12, 24, and 72 h (8 each ischemia; 2 each sham) groups of animals were evaluated on a 28 point clinical scale, blood and CSF obtained, and the brains were evaluated for infarct volume and IL-6 mRNA levels. Serum levels of IL-6 (ELISA mean ± SD; undetectable in controls) overall sham group, 102 ± 87; 3 h, 908 ± 494 * pg ml-1; 6 h, 1079 ± 468 * pg ml- 1; 12 h, 980 ± 221 * pg ml-1; 24 h, 320 ± 314 * pg ml-1; 72 h, 20 ± 30 * pg ml-1 (* p <0.05 to sham). CSF levels (ELISA) overall sham group, 10 ± 18; 3 h, 379 ± 210 * pg ml-1; 6 h, 157 ± 61 * pg ml-1; 12 h, 136 ± 88 * pg ml-1; 24 h, 127 ± 99 pg ml-1; 72 h, 72 ± 9 * pg ml- 1 (* p ≤ 0.05 to sham). Brain IL-6 mRNA levels overall sham group, 20; 3 h, 480; 6 h, 599; 12 h, 7960; 24 h, 20267; 72 h, 0. There was an overall R2 of 0.20 between plasma and CSF IL-6. There was an overall R2 of 0.13 and 0.20 between infarct size and serum and CSF IL-6 level respectively, and an overall R2 of 0.10 and 0.17 between neurologic function and serum and CSF IL-6 level respectively. These findings confirm that IL-6 values increase following CNS ischemia with peak serum and CSF levels occurring before brain values. CSF IL-6 levels had a stronger correlation with neurologic function and infarct size than serum.

KW - CNS ischemia

KW - Infarct size

KW - Inflammatory response

KW - Interleukin-6

KW - Neurologic function

UR - http://www.scopus.com/inward/record.url?scp=0032915929&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032915929&partnerID=8YFLogxK

M3 - Article

C2 - 10319338

AN - SCOPUS:0032915929

VL - 21

SP - 287

EP - 292

JO - Neurological Research

JF - Neurological Research

SN - 0161-6412

IS - 3

ER -