Time course of growth factor staining in a rabbit model of traumatic tractional retinal detachment

Igor Westra, Susan G. Robbins, David Wilson, Joseph Robertson, Leslie M. O'Rourke, James (Jim) Rosenbaum, Charles E. Hart

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

•Background: This study examined the relationship between growth factor expression and cellular proliferation during the evolution of traumatic tractional retinal detachment (TRD) in a rabbit model. •Methods: TRD was induced in 15 pigmented rabbits by treating the inferior retina with cryopexy and making a scleral incision superiorly. Sections from varied time points were stained in the same assay with mouse monoclonal antibodies (MAb) specific for basic fibroblastic growth factor (bFGF) and platelet-derived growth factor (PDGF-BB/AB). • Results: Initially, the eyes exhibited intense vitritis; discrete membranes were present at 7 days and progressed to tractional retinal detachment at 17 and 28 days, after which there was no clinical change. At 6 and 24 h, bFGF, PDGF, and proliferating cell nuclear antigen (PCNA) were not detectable in membranes or wound sites (except for PDGF-positive in flammatory cells). On days 7, 17, 28, and 52, bFGF and PDGF were readily detectable in most membranes. Cellular proliferation as detected by PCNA staining was also present on days 7, 17, and 28, but was absent by day 52 despite growth factor staining. At all times, PCNA staining, which was most intense at the wound site, showed only limited correlation with staining for either growth factor for individual cells. Müller cells stained positively for PDGF-BB/AB in 13 of the 15 TRD eyes, but in none of the normal eyes. • Conclusions: Since cellular proliferation correlated incompletely with the staining for bFGF and PDGF, these growth factors may not account exclusively for cellular proliferation within the membrane. Their distribution, however, including PDGF staining of Müller cells and bFGF staining at the vitreous-membrane interface, suggests that they may have roles in the pathogenesis of TRD.

Original languageEnglish (US)
Pages (from-to)573-581
Number of pages9
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume233
Issue number9
DOIs
StatePublished - Sep 1995

Fingerprint

Retinal Detachment
Intercellular Signaling Peptides and Proteins
Staining and Labeling
Rabbits
Proliferating Cell Nuclear Antigen
Membranes
Cell Proliferation
Wounds and Injuries
Retina
Monoclonal Antibodies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Time course of growth factor staining in a rabbit model of traumatic tractional retinal detachment. / Westra, Igor; Robbins, Susan G.; Wilson, David; Robertson, Joseph; O'Rourke, Leslie M.; Rosenbaum, James (Jim); Hart, Charles E.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 233, No. 9, 09.1995, p. 573-581.

Research output: Contribution to journalArticle

Westra, Igor ; Robbins, Susan G. ; Wilson, David ; Robertson, Joseph ; O'Rourke, Leslie M. ; Rosenbaum, James (Jim) ; Hart, Charles E. / Time course of growth factor staining in a rabbit model of traumatic tractional retinal detachment. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 1995 ; Vol. 233, No. 9. pp. 573-581.
@article{df1c904e499b4e979ac834940a1223d0,
title = "Time course of growth factor staining in a rabbit model of traumatic tractional retinal detachment",
abstract = "•Background: This study examined the relationship between growth factor expression and cellular proliferation during the evolution of traumatic tractional retinal detachment (TRD) in a rabbit model. •Methods: TRD was induced in 15 pigmented rabbits by treating the inferior retina with cryopexy and making a scleral incision superiorly. Sections from varied time points were stained in the same assay with mouse monoclonal antibodies (MAb) specific for basic fibroblastic growth factor (bFGF) and platelet-derived growth factor (PDGF-BB/AB). • Results: Initially, the eyes exhibited intense vitritis; discrete membranes were present at 7 days and progressed to tractional retinal detachment at 17 and 28 days, after which there was no clinical change. At 6 and 24 h, bFGF, PDGF, and proliferating cell nuclear antigen (PCNA) were not detectable in membranes or wound sites (except for PDGF-positive in flammatory cells). On days 7, 17, 28, and 52, bFGF and PDGF were readily detectable in most membranes. Cellular proliferation as detected by PCNA staining was also present on days 7, 17, and 28, but was absent by day 52 despite growth factor staining. At all times, PCNA staining, which was most intense at the wound site, showed only limited correlation with staining for either growth factor for individual cells. M{\"u}ller cells stained positively for PDGF-BB/AB in 13 of the 15 TRD eyes, but in none of the normal eyes. • Conclusions: Since cellular proliferation correlated incompletely with the staining for bFGF and PDGF, these growth factors may not account exclusively for cellular proliferation within the membrane. Their distribution, however, including PDGF staining of M{\"u}ller cells and bFGF staining at the vitreous-membrane interface, suggests that they may have roles in the pathogenesis of TRD.",
author = "Igor Westra and Robbins, {Susan G.} and David Wilson and Joseph Robertson and O'Rourke, {Leslie M.} and Rosenbaum, {James (Jim)} and Hart, {Charles E.}",
year = "1995",
month = "9",
doi = "10.1007/BF00404709",
language = "English (US)",
volume = "233",
pages = "573--581",
journal = "Albrecht von Graefes Archiv für Klinische und Experimentelle Ophthalmologie",
issn = "0065-6100",
publisher = "Springer Verlag",
number = "9",

}

TY - JOUR

T1 - Time course of growth factor staining in a rabbit model of traumatic tractional retinal detachment

AU - Westra, Igor

AU - Robbins, Susan G.

AU - Wilson, David

AU - Robertson, Joseph

AU - O'Rourke, Leslie M.

AU - Rosenbaum, James (Jim)

AU - Hart, Charles E.

PY - 1995/9

Y1 - 1995/9

N2 - •Background: This study examined the relationship between growth factor expression and cellular proliferation during the evolution of traumatic tractional retinal detachment (TRD) in a rabbit model. •Methods: TRD was induced in 15 pigmented rabbits by treating the inferior retina with cryopexy and making a scleral incision superiorly. Sections from varied time points were stained in the same assay with mouse monoclonal antibodies (MAb) specific for basic fibroblastic growth factor (bFGF) and platelet-derived growth factor (PDGF-BB/AB). • Results: Initially, the eyes exhibited intense vitritis; discrete membranes were present at 7 days and progressed to tractional retinal detachment at 17 and 28 days, after which there was no clinical change. At 6 and 24 h, bFGF, PDGF, and proliferating cell nuclear antigen (PCNA) were not detectable in membranes or wound sites (except for PDGF-positive in flammatory cells). On days 7, 17, 28, and 52, bFGF and PDGF were readily detectable in most membranes. Cellular proliferation as detected by PCNA staining was also present on days 7, 17, and 28, but was absent by day 52 despite growth factor staining. At all times, PCNA staining, which was most intense at the wound site, showed only limited correlation with staining for either growth factor for individual cells. Müller cells stained positively for PDGF-BB/AB in 13 of the 15 TRD eyes, but in none of the normal eyes. • Conclusions: Since cellular proliferation correlated incompletely with the staining for bFGF and PDGF, these growth factors may not account exclusively for cellular proliferation within the membrane. Their distribution, however, including PDGF staining of Müller cells and bFGF staining at the vitreous-membrane interface, suggests that they may have roles in the pathogenesis of TRD.

AB - •Background: This study examined the relationship between growth factor expression and cellular proliferation during the evolution of traumatic tractional retinal detachment (TRD) in a rabbit model. •Methods: TRD was induced in 15 pigmented rabbits by treating the inferior retina with cryopexy and making a scleral incision superiorly. Sections from varied time points were stained in the same assay with mouse monoclonal antibodies (MAb) specific for basic fibroblastic growth factor (bFGF) and platelet-derived growth factor (PDGF-BB/AB). • Results: Initially, the eyes exhibited intense vitritis; discrete membranes were present at 7 days and progressed to tractional retinal detachment at 17 and 28 days, after which there was no clinical change. At 6 and 24 h, bFGF, PDGF, and proliferating cell nuclear antigen (PCNA) were not detectable in membranes or wound sites (except for PDGF-positive in flammatory cells). On days 7, 17, 28, and 52, bFGF and PDGF were readily detectable in most membranes. Cellular proliferation as detected by PCNA staining was also present on days 7, 17, and 28, but was absent by day 52 despite growth factor staining. At all times, PCNA staining, which was most intense at the wound site, showed only limited correlation with staining for either growth factor for individual cells. Müller cells stained positively for PDGF-BB/AB in 13 of the 15 TRD eyes, but in none of the normal eyes. • Conclusions: Since cellular proliferation correlated incompletely with the staining for bFGF and PDGF, these growth factors may not account exclusively for cellular proliferation within the membrane. Their distribution, however, including PDGF staining of Müller cells and bFGF staining at the vitreous-membrane interface, suggests that they may have roles in the pathogenesis of TRD.

UR - http://www.scopus.com/inward/record.url?scp=0029101830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029101830&partnerID=8YFLogxK

U2 - 10.1007/BF00404709

DO - 10.1007/BF00404709

M3 - Article

C2 - 8543209

AN - SCOPUS:0029101830

VL - 233

SP - 573

EP - 581

JO - Albrecht von Graefes Archiv für Klinische und Experimentelle Ophthalmologie

JF - Albrecht von Graefes Archiv für Klinische und Experimentelle Ophthalmologie

SN - 0065-6100

IS - 9

ER -