TY - JOUR
T1 - Thyrotropin-releasing hormone (TRH) content of rat striatum
T2 - Modification by drugs and lesions
AU - Spindel, Eliot R.
AU - Pettibone, Douglas J.
AU - Wurtman, Richard J.
N1 - Funding Information:
These studies were supported by grants from the International Life Sciences Institute and the National Institutes of Health. We thank Dr. Franz Hefti for advice on stereotaxic procedures, Dr. Arthur Schlossberg for performing the raphe lesions and Dr. Alan Sved for the serotonin analysis.
PY - 1981/7/20
Y1 - 1981/7/20
N2 - Two hours after injection, d-amphetamine sulfate (10 mg/kg, i.p.) lowered thyrotropin-releasing hormone (TRH) levels in rat striatum by 50%, but produced no significant changes in the TRH contents of hypothalamus, septum, brain stem or preoptic area. The effect peaked 2 h after amphetamine injection and declined slowly thereafter. The amphetamine-induced decrease in striatal TRH could be blocked by pretreatment with haloperidol or α-methyltyrosine, or by production of a 6-hydroxydopamine lesion in the ipsilateral substantia nigra. Amphetamine did not act by inhibiting protein synthesis in as much as cycloheximide did not similarly decrease striatal TRH. Kainic acid injected into the striatum lowered TRH by 30% after 5 days. In contrast, partial deafferentation of the striatum (by cerebral hemitransection at mid-hypothalamic level) increased striatal TRH 2-3-fold, while lesions of the dorsal raphe did not significantly change striatal TRH. Thus TRH levels in rat striatum are closely regulated by dopaminergic and other neurotransmitter systems.
AB - Two hours after injection, d-amphetamine sulfate (10 mg/kg, i.p.) lowered thyrotropin-releasing hormone (TRH) levels in rat striatum by 50%, but produced no significant changes in the TRH contents of hypothalamus, septum, brain stem or preoptic area. The effect peaked 2 h after amphetamine injection and declined slowly thereafter. The amphetamine-induced decrease in striatal TRH could be blocked by pretreatment with haloperidol or α-methyltyrosine, or by production of a 6-hydroxydopamine lesion in the ipsilateral substantia nigra. Amphetamine did not act by inhibiting protein synthesis in as much as cycloheximide did not similarly decrease striatal TRH. Kainic acid injected into the striatum lowered TRH by 30% after 5 days. In contrast, partial deafferentation of the striatum (by cerebral hemitransection at mid-hypothalamic level) increased striatal TRH 2-3-fold, while lesions of the dorsal raphe did not significantly change striatal TRH. Thus TRH levels in rat striatum are closely regulated by dopaminergic and other neurotransmitter systems.
KW - amphetamine
KW - dopamine
KW - striatum
KW - thyrotropin-releasing hormone
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U2 - 10.1016/0006-8993(81)90134-7
DO - 10.1016/0006-8993(81)90134-7
M3 - Article
C2 - 6265032
AN - SCOPUS:0019416586
SN - 0006-8993
VL - 216
SP - 323
EP - 331
JO - Brain research
JF - Brain research
IS - 2
ER -