Thymic Origin of the Prolactin-Dependent Nb2 Lymphoma Cell Line

William Fleming, Robert J. Matusik, Henry G. Friesen, Norman M. Pettigrew

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Blast cells in acute leukemia and lymphoma appear to be “frozen” at various stages of lymphoid cell differentiation. The enzymatic and antigenic phenotypes expressed by these cells often correspond to the gene products of their normal precursors. We have used various immunocytochemical and enzymatic techniques to identify membrane, nuclear, and cytoplasmic markers associated with the prolactin-dependent Nb2 lymphoma cell line. The Nb2 cells, whether stationary or in log-phase growth, did not express any surface immunoglobulin. However, 100% of the Nb2 cells bound both a monoclonal antibody raised to rat thymocyte W3/25-HLK, which specifically binds an antigenic determinant on rat T-helper cells, and second monoclonal antibody 0X8-HL, which identifies rat nonhelper T-cells. Transmission electron microscopy showed no evidence of phagocytic vacuoles, and activity of the lysosomal enzyme muramidase was also absent. There was no evidence of the DNA polymerase enzyme terminal deoxynucleotidyl transferase. ±-Naphthyl acetate esterase activity was indicated in about 50% of the Nb2 cells by a faint particulate cytoplasmic staining similar to that found in thymocytes. Rosette formation with guinea pig erythrocytes, a property of mature rat thymocytes, was not observed with Nb2 cells. The data suggest that the Nb2 tumor may have arisen from a thymocyte at an intermediate stage of differentiation. The presence of Thy-like ±-naphthyl acetate esterase pattern and the binding of both W3/25-HLK and 0X8-HL support the thymic origin and relative immaturity of these lymphoid cells. It is becoming increasingly apparent that a significant proportion of lymphomas and leukemias also originate in undifferentiated thymic cells.

Original languageEnglish (US)
Pages (from-to)3138-3141
Number of pages4
JournalCancer Research
Volume42
Issue number8
StatePublished - Aug 1 1982
Externally publishedYes

Fingerprint

Prolactin
Lymphoma
Cell Line
Thymocytes
Acetylesterase
Leukemia
Monoclonal Antibodies
Lymphocytes
Rosette Formation
B-Cell Antigen Receptors
DNA Nucleotidylexotransferase
Nuclear Envelope
DNA-Directed DNA Polymerase
Enzymes
Muramidase
Helper-Inducer T-Lymphocytes
Vacuoles
Transmission Electron Microscopy
Epitopes
Cell Differentiation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fleming, W., Matusik, R. J., Friesen, H. G., & Pettigrew, N. M. (1982). Thymic Origin of the Prolactin-Dependent Nb2 Lymphoma Cell Line. Cancer Research, 42(8), 3138-3141.

Thymic Origin of the Prolactin-Dependent Nb2 Lymphoma Cell Line. / Fleming, William; Matusik, Robert J.; Friesen, Henry G.; Pettigrew, Norman M.

In: Cancer Research, Vol. 42, No. 8, 01.08.1982, p. 3138-3141.

Research output: Contribution to journalArticle

Fleming, W, Matusik, RJ, Friesen, HG & Pettigrew, NM 1982, 'Thymic Origin of the Prolactin-Dependent Nb2 Lymphoma Cell Line', Cancer Research, vol. 42, no. 8, pp. 3138-3141.
Fleming W, Matusik RJ, Friesen HG, Pettigrew NM. Thymic Origin of the Prolactin-Dependent Nb2 Lymphoma Cell Line. Cancer Research. 1982 Aug 1;42(8):3138-3141.
Fleming, William ; Matusik, Robert J. ; Friesen, Henry G. ; Pettigrew, Norman M. / Thymic Origin of the Prolactin-Dependent Nb2 Lymphoma Cell Line. In: Cancer Research. 1982 ; Vol. 42, No. 8. pp. 3138-3141.
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