Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission

Thao Do, Gavin Murphy, Lesley A. Earl, Gregory Q. Del Prete, Giovanna Grandinetti, Guan Han Li, Jacob Estes, Prashant Rao, Charles M. Trubey, James Thomas, Jeffrey Spector, Donald Bliss, Avindra Nath, Jeffrey D. Lifson, Sriram Subramaniam

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

HIV transmission efficiency is greatly increased when viruses are transmitted at virological synapses formed between infected and uninfected cells. We have previously shown that virological synapses formed between HIV-pulsed mature dendritic cells (DCs) and uninfected T cells contain interdigitated membrane surfaces, with T cell filopodia extending toward virions sequestered deep inside invaginations formed on the DC membrane. To explore membrane structural changes relevant to HIV transmission across other types of intercellular conjugates, we used a combination of light and focused ion beam scanning electron microscopy (FIB-SEM) to determine the three-dimensional (3D) architectures of contact regions between HIV-1-infected CD4+ T cells and either uninfected human CD4+ T cells or human fetal astrocytes. We present evidence that in each case, membrane extensions that originate from the uninfected cells, either as membrane sheets or filopodial bridges, are present and may be involved in HIV transmission from infected to uninfected cells. We show that individual virions are distributed along the length of astrocyte filopodia, suggesting that virus transfer to the astrocytes is mediated, at least in part, by processes originating from the astrocyte itself. Mechanisms that selectively disrupt the polarization and formation of such membrane extensions could thus represent a possible target for reducing viral spread.

Original languageEnglish (US)
Pages (from-to)10327-10339
Number of pages13
JournalJournal of Virology
Volume88
Issue number18
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Three-Dimensional Imaging
astrocytes
virus transmission
synapse
Human immunodeficiency virus 1
Synapses
HIV-1
T-lymphocytes
Astrocytes
image analysis
pseudopodia
Viruses
Membranes
HIV
T-Lymphocytes
dendritic cells
virion
Pseudopodia
Virion
Dendritic Cells

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Do, T., Murphy, G., Earl, L. A., Del Prete, G. Q., Grandinetti, G., Li, G. H., ... Subramaniam, S. (2014). Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission. Journal of Virology, 88(18), 10327-10339. https://doi.org/10.1128/JVI.00788-14

Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission. / Do, Thao; Murphy, Gavin; Earl, Lesley A.; Del Prete, Gregory Q.; Grandinetti, Giovanna; Li, Guan Han; Estes, Jacob; Rao, Prashant; Trubey, Charles M.; Thomas, James; Spector, Jeffrey; Bliss, Donald; Nath, Avindra; Lifson, Jeffrey D.; Subramaniam, Sriram.

In: Journal of Virology, Vol. 88, No. 18, 01.01.2014, p. 10327-10339.

Research output: Contribution to journalArticle

Do, T, Murphy, G, Earl, LA, Del Prete, GQ, Grandinetti, G, Li, GH, Estes, J, Rao, P, Trubey, CM, Thomas, J, Spector, J, Bliss, D, Nath, A, Lifson, JD & Subramaniam, S 2014, 'Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission', Journal of Virology, vol. 88, no. 18, pp. 10327-10339. https://doi.org/10.1128/JVI.00788-14
Do, Thao ; Murphy, Gavin ; Earl, Lesley A. ; Del Prete, Gregory Q. ; Grandinetti, Giovanna ; Li, Guan Han ; Estes, Jacob ; Rao, Prashant ; Trubey, Charles M. ; Thomas, James ; Spector, Jeffrey ; Bliss, Donald ; Nath, Avindra ; Lifson, Jeffrey D. ; Subramaniam, Sriram. / Three-dimensional imaging of HIV-1 virological synapses reveals membrane architectures involved in virus transmission. In: Journal of Virology. 2014 ; Vol. 88, No. 18. pp. 10327-10339.
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