Thiourethane-functionalized fillers: Biological properties and degradation resistance

Larissa Maria Cavalcante, Amanda Barreto Ramos, Daniela Costa Silva, Gutemberg Gomes Alves, Karinne Bueno Antunes, Carmem Silvia Pfeifer, Luis Felipe Jochims Schneider

Research output: Contribution to journalArticlepeer-review


This study determined the effect of thiourethane-functionalized fillers (TU) on the antimicrobial properties, cytotoxicity, degree of conversion (DC), water sorption (Wsp) and solubility (Wsl) of experimental composites. TU-modified fillers were added at different ratios in experimental composites: 0 (Control-TU0), 25% (TU25), 50% (TU50), 75% (TU75) and 100wt% (TU100). The antimicrobial properties were detected through the exhaustion test and counting of Streptococus mutanscolonies for biofilm formation. Cytotoxicity to human gingival fibroblasts was evaluated in three different parameters: XTT (2,3-Bis- (2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide), NRU (Neutral Red Uptake assay) and CVDE (Crystal Violet Dye Exclusion test)) at the same cells. ELISA was used to measure the IL-6 and b-FGF biomarkers. DC was determined by Fourier-transformed infrared spectroscopy, while Wsp and Wsl by mass variations. Inhibitory capacity of biofilm formation was not observed for any material. All groups presented at least 70% of cell survival within the observed periods (24h and 7 days). Positive control (toxic) had high IL-6 values and low b-FGF values. No significant variations in DC, Wsp, and Wsl were observed among the experimental groups. The use of thiourethane did not present antimicrobial and cytotoxic activity and the tested materials presented equivalent properties to those conventionally used in dentistry.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalBrazilian oral research
StatePublished - 2021


  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Biomarkers
  • Materials Testing

ASJC Scopus subject areas

  • Dentistry(all)


Dive into the research topics of 'Thiourethane-functionalized fillers: Biological properties and degradation resistance'. Together they form a unique fingerprint.

Cite this