TY - JOUR
T1 - Thiourethane-functionalized fillers
T2 - Biological properties and degradation resistance
AU - Cavalcante, Larissa Maria
AU - Ramos, Amanda Barreto
AU - Silva, Daniela Costa
AU - Alves, Gutemberg Gomes
AU - Antunes, Karinne Bueno
AU - Pfeifer, Carmem Silvia
AU - Schneider, Luis Felipe Jochims
N1 - Funding Information:
The authors dedicate this article to the memory of Prof. Dr. Raphael Hirata Junior, from the School of Medical Sciences (UERJ), who performed the antimicrobial assay. This article involves part of the results from the AB PhD thesis. LMC and LFS are grateful to Funadesp for research scholarships. LFS is grateful to Faperj for research scholarship (JCNE E-26/202.735/2018).
Funding Information:
of Prof. Dr. Raphael Hirata Junior, from the School of Medical Sciences (UERJ), who performed the antimicrobial assay. This article involves part of the results from the AB PhD thesis. LMC and LFS are grateful to Funadesp for research scholarships. LFS is grateful to Faperj for research scholarship (JCNE E-26/202.735/2018).
Publisher Copyright:
© 2021
PY - 2021
Y1 - 2021
N2 - This study determined the effect of thiourethane-functionalized fillers (TU) on the antimicrobial properties, cytotoxicity, degree of conversion (DC), water sorption (Wsp) and solubility (Wsl) of experimental composites. TU-modified fillers were added at different ratios in experimental composites: 0 (Control-TU0), 25% (TU25), 50% (TU50), 75% (TU75) and 100wt% (TU100). The antimicrobial properties were detected through the exhaustion test and counting of Streptococus mutanscolonies for biofilm formation. Cytotoxicity to human gingival fibroblasts was evaluated in three different parameters: XTT (2,3-Bis- (2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide), NRU (Neutral Red Uptake assay) and CVDE (Crystal Violet Dye Exclusion test)) at the same cells. ELISA was used to measure the IL-6 and b-FGF biomarkers. DC was determined by Fourier-transformed infrared spectroscopy, while Wsp and Wsl by mass variations. Inhibitory capacity of biofilm formation was not observed for any material. All groups presented at least 70% of cell survival within the observed periods (24h and 7 days). Positive control (toxic) had high IL-6 values and low b-FGF values. No significant variations in DC, Wsp, and Wsl were observed among the experimental groups. The use of thiourethane did not present antimicrobial and cytotoxic activity and the tested materials presented equivalent properties to those conventionally used in dentistry.
AB - This study determined the effect of thiourethane-functionalized fillers (TU) on the antimicrobial properties, cytotoxicity, degree of conversion (DC), water sorption (Wsp) and solubility (Wsl) of experimental composites. TU-modified fillers were added at different ratios in experimental composites: 0 (Control-TU0), 25% (TU25), 50% (TU50), 75% (TU75) and 100wt% (TU100). The antimicrobial properties were detected through the exhaustion test and counting of Streptococus mutanscolonies for biofilm formation. Cytotoxicity to human gingival fibroblasts was evaluated in three different parameters: XTT (2,3-Bis- (2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide), NRU (Neutral Red Uptake assay) and CVDE (Crystal Violet Dye Exclusion test)) at the same cells. ELISA was used to measure the IL-6 and b-FGF biomarkers. DC was determined by Fourier-transformed infrared spectroscopy, while Wsp and Wsl by mass variations. Inhibitory capacity of biofilm formation was not observed for any material. All groups presented at least 70% of cell survival within the observed periods (24h and 7 days). Positive control (toxic) had high IL-6 values and low b-FGF values. No significant variations in DC, Wsp, and Wsl were observed among the experimental groups. The use of thiourethane did not present antimicrobial and cytotoxic activity and the tested materials presented equivalent properties to those conventionally used in dentistry.
KW - Anti-Bacterial Agents
KW - Anti-Infective Agents
KW - Biomarkers
KW - Materials Testing
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U2 - 10.1590/1807-3107BOR-2021.VOL35.0018
DO - 10.1590/1807-3107BOR-2021.VOL35.0018
M3 - Article
C2 - 33331409
AN - SCOPUS:85098605781
SN - 1806-8324
VL - 35
SP - 1
EP - 10
JO - Pesquisa odontologica brasileira = Brazilian oral research
JF - Pesquisa odontologica brasileira = Brazilian oral research
ER -