Thiol Quantification Using Colorimetric Thiol-Disulfide Exchange in Nonaqueous Solvents

Ryan Schmidt; Matthew G. Logan; Sarah Patty; Jack L. Ferracane; Carmem S. Pfeifer; Alexander J. Kendall

doi:10.1021/acsomega.2c07792

Thiol Quantification Using Colorimetric Thiol-Disulfide Exchange in Nonaqueous Solvents

Ryan Schmidt, Matthew G. Logan, Sarah Patty, Jack L. Ferracane, Carmem S. Pfeifer, Alexander J. Kendall

School of Dentistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A careful analysis of two (thiol-disulfide exchange) thiol quantification chromophores’ behavior (Ellman’s reagent and Aldrithiol-4) in nonaqueous solvents is presented. A wide range of kinetic profiles and response factors were measured to exhibit a large variance for nonaqueous systems. We report several robust benchtop and room-temperature methods using different organic solvents compared to aqueous conditions. Validation of analytical analyses in nonaqueous systems and quantification of the cysteine content of ovalbumin are also presented. This work serves as a treatise on the utilization of thiol-disulfide exchange chromophores under nonaqueous conditions for the quantification of thiols.

Original language	English (US)
Pages (from-to)	9356-9363
Number of pages	8
Journal	ACS Omega
Volume	8
Issue number	10
DOIs	https://doi.org/10.1021/acsomega.2c07792
State	Published - Mar 14 2023

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

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10.1021/acsomega.2c07792

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title = "Thiol Quantification Using Colorimetric Thiol-Disulfide Exchange in Nonaqueous Solvents",

abstract = "A careful analysis of two (thiol-disulfide exchange) thiol quantification chromophores{\textquoteright} behavior (Ellman{\textquoteright}s reagent and Aldrithiol-4) in nonaqueous solvents is presented. A wide range of kinetic profiles and response factors were measured to exhibit a large variance for nonaqueous systems. We report several robust benchtop and room-temperature methods using different organic solvents compared to aqueous conditions. Validation of analytical analyses in nonaqueous systems and quantification of the cysteine content of ovalbumin are also presented. This work serves as a treatise on the utilization of thiol-disulfide exchange chromophores under nonaqueous conditions for the quantification of thiols.",

author = "Ryan Schmidt and Logan, {Matthew G.} and Sarah Patty and Ferracane, {Jack L.} and Pfeifer, {Carmem S.} and Kendall, {Alexander J.}",

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T1 - Thiol Quantification Using Colorimetric Thiol-Disulfide Exchange in Nonaqueous Solvents

AU - Schmidt, Ryan

AU - Logan, Matthew G.

AU - Patty, Sarah

AU - Ferracane, Jack L.

AU - Pfeifer, Carmem S.

AU - Kendall, Alexander J.

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Y1 - 2023/3/14

N2 - A careful analysis of two (thiol-disulfide exchange) thiol quantification chromophores’ behavior (Ellman’s reagent and Aldrithiol-4) in nonaqueous solvents is presented. A wide range of kinetic profiles and response factors were measured to exhibit a large variance for nonaqueous systems. We report several robust benchtop and room-temperature methods using different organic solvents compared to aqueous conditions. Validation of analytical analyses in nonaqueous systems and quantification of the cysteine content of ovalbumin are also presented. This work serves as a treatise on the utilization of thiol-disulfide exchange chromophores under nonaqueous conditions for the quantification of thiols.

AB - A careful analysis of two (thiol-disulfide exchange) thiol quantification chromophores’ behavior (Ellman’s reagent and Aldrithiol-4) in nonaqueous solvents is presented. A wide range of kinetic profiles and response factors were measured to exhibit a large variance for nonaqueous systems. We report several robust benchtop and room-temperature methods using different organic solvents compared to aqueous conditions. Validation of analytical analyses in nonaqueous systems and quantification of the cysteine content of ovalbumin are also presented. This work serves as a treatise on the utilization of thiol-disulfide exchange chromophores under nonaqueous conditions for the quantification of thiols.

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Thiol Quantification Using Colorimetric Thiol-Disulfide Exchange in Nonaqueous Solvents

Abstract

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