Thick-section fluorescence in situ hybridization on formalin-fixed, paraffin-embedded archival tissue provides a histogenetic profile

Curtis T. Thompson, Philip E. LeBoit, Petra M. Nederlof, Joe Gray

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Fluorescence in situ hybridization has become a major tool for analysis of gene and chromosome copy number in normal and malignant tissue. The technique has been applied widely to fresh tissue and dispersed formalin-fixed, paraffin-embedded archival tissue, but its use on sections of archival tissue has largely been limited to sections 20 μ thick that overcomes these difficulties. Key developments were the use of DNA probes directly labeled with fluorochromes and optical sectioning using laser- scanning confocal microscopy.

Original languageEnglish (US)
Pages (from-to)237-243
Number of pages7
JournalAmerican Journal of Pathology
Volume144
Issue number2
StatePublished - Feb 1994
Externally publishedYes

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Fluorescence In Situ Hybridization
Paraffin
Formaldehyde
Gene Dosage
DNA Probes
Fluorescent Dyes
Confocal Microscopy
Chromosomes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Thick-section fluorescence in situ hybridization on formalin-fixed, paraffin-embedded archival tissue provides a histogenetic profile. / Thompson, Curtis T.; LeBoit, Philip E.; Nederlof, Petra M.; Gray, Joe.

In: American Journal of Pathology, Vol. 144, No. 2, 02.1994, p. 237-243.

Research output: Contribution to journalArticle

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