Thermal stability of a long-acting analogue of luteinizing hormone releasing hormone (D-trp6-pro9-NEt-LHRH)

Jorg Winterer, Dulal Chatterji, Florence Comite, Marshall H. Decker, D. Lynn Loriaux, Joseph F. Gallelli, Gordon B. Cutler

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The stability of solutions of a long-acting analogue of luteinizing hormone releasing hormone (D-trp6-pro9-NEt-LHRH [LHRHa] after heating to 60C for 5 days, after repeated freezing and thawing, and after refrigeration at 4C for 8 days has been examined. None of the treatments caused a detectable alteration in the HPLC profile, and none caused a significant change in biological activity in vivo. It is concluded that D-trp6-pro9-NEt-LHRH can withstand mild heating, repeated freezing and thawing, and short-term refrigeration without apparent change in HPLC profile or biological activity. It is also concluded that the results obtained with the HPLC method correlate well with the results from the in vivo bioassay.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalContraception
Volume27
Issue number2
DOIs
StatePublished - Feb 1983

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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