Thermal stability of a long-acting analogue of luteinizing hormone releasing hormone (D-trp6-pro9-NEt-LHRH)

Jorg Winterer; Dulal Chatterji; Florence Comite; Marshall H. Decker; D. Lynn Loriaux; Joseph F. Gallelli; Gordon B. Cutler

doi:10.1016/0010-7824(83)90089-6

Thermal stability of a long-acting analogue of luteinizing hormone releasing hormone (D-trp⁶-pro₉-NEt-LHRH)

Jorg Winterer, Dulal Chatterji, Florence Comite, Marshall H. Decker, D. Lynn Loriaux, Joseph F. Gallelli, Gordon B. Cutler

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The stability of solutions of a long-acting analogue of luteinizing hormone releasing hormone (D-trp⁶-pro⁹-NEt-LHRH [LHRH_a] after heating to 60C for 5 days, after repeated freezing and thawing, and after refrigeration at 4C for 8 days has been examined. None of the treatments caused a detectable alteration in the HPLC profile, and none caused a significant change in biological activity in vivo. It is concluded that D-trp⁶-pro⁹-NEt-LHRH can withstand mild heating, repeated freezing and thawing, and short-term refrigeration without apparent change in HPLC profile or biological activity. It is also concluded that the results obtained with the HPLC method correlate well with the results from the in vivo bioassay.

Original language	English (US)
Pages (from-to)	195-200
Number of pages	6
Journal	Contraception
Volume	27
Issue number	2
DOIs	https://doi.org/10.1016/0010-7824(83)90089-6
State	Published - Feb 1983
Externally published	Yes

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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10.1016/0010-7824(83)90089-6

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title = "Thermal stability of a long-acting analogue of luteinizing hormone releasing hormone (D-trp6-pro9-NEt-LHRH)",

abstract = "The stability of solutions of a long-acting analogue of luteinizing hormone releasing hormone (D-trp6-pro9-NEt-LHRH [LHRHa] after heating to 60C for 5 days, after repeated freezing and thawing, and after refrigeration at 4C for 8 days has been examined. None of the treatments caused a detectable alteration in the HPLC profile, and none caused a significant change in biological activity in vivo. It is concluded that D-trp6-pro9-NEt-LHRH can withstand mild heating, repeated freezing and thawing, and short-term refrigeration without apparent change in HPLC profile or biological activity. It is also concluded that the results obtained with the HPLC method correlate well with the results from the in vivo bioassay.",

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AU - Winterer, Jorg

AU - Chatterji, Dulal

AU - Comite, Florence

AU - Decker, Marshall H.

AU - Loriaux, D. Lynn

AU - Gallelli, Joseph F.

AU - Cutler, Gordon B.

PY - 1983/2

Y1 - 1983/2

N2 - The stability of solutions of a long-acting analogue of luteinizing hormone releasing hormone (D-trp6-pro9-NEt-LHRH [LHRHa] after heating to 60C for 5 days, after repeated freezing and thawing, and after refrigeration at 4C for 8 days has been examined. None of the treatments caused a detectable alteration in the HPLC profile, and none caused a significant change in biological activity in vivo. It is concluded that D-trp6-pro9-NEt-LHRH can withstand mild heating, repeated freezing and thawing, and short-term refrigeration without apparent change in HPLC profile or biological activity. It is also concluded that the results obtained with the HPLC method correlate well with the results from the in vivo bioassay.

AB - The stability of solutions of a long-acting analogue of luteinizing hormone releasing hormone (D-trp6-pro9-NEt-LHRH [LHRHa] after heating to 60C for 5 days, after repeated freezing and thawing, and after refrigeration at 4C for 8 days has been examined. None of the treatments caused a detectable alteration in the HPLC profile, and none caused a significant change in biological activity in vivo. It is concluded that D-trp6-pro9-NEt-LHRH can withstand mild heating, repeated freezing and thawing, and short-term refrigeration without apparent change in HPLC profile or biological activity. It is also concluded that the results obtained with the HPLC method correlate well with the results from the in vivo bioassay.

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Thermal stability of a long-acting analogue of luteinizing hormone releasing hormone (D-trp⁶-pro₉-NEt-LHRH)

Abstract

ASJC Scopus subject areas

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