Therapeutic ultrasound promotes reperfusion and angiogenesis in a rat model of peripheral arterial disease

Babak Nazer, Farzin Ghahghaie, Risa Kashima, Tatiana Khokhlova, Camilo Perez, Lawrence Crum, Tom Matula, Akiko Hata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Shock wave therapy (SWT) is an acoustic technology clinically used for the non-invasive treatment of ischemic heart disease (IHD). Therapeutic ultrasound (TUS) has more recently been developed for the same indication, although its effects on reperfusion and angiogenesis have yet to be directly compared to those of SWT. Methods and Results: TUS and SWT acoustic parameters were matched, and their ability to promote angiogenesis and reperfusion in a rat hindlimb ischemia model was compared. After left femoral artery excision, 3-weekly TUS, SWT or sham treatments (n=10 rats each) of the left hindlimb were performed for 2 weeks. Laser Doppler perfusion imaging demonstrated improved perfusion with TUS (66±4% L:R hindlimb perfusion, mean ± SEM, P=0.02), but not with SWT (59±4%, P=0.13) compared with sham (50±4%). Immunohistochemistry of CD31 demonstrated increased microvascular density with TUS (222.6 vessels/high-power field, P=0.001) and SWT (216.9, P=0.01) compared to sham-treated rats (196.0). Tissue vascular endothelial growth factor mRNA levels were elevated in the left hindlimb of TUS-, but not SWT- or sham-treated rats. Conclusions: Direct comparison demonstrates that TUS is more effective than SWT at promoting reperfusion, whereas both therapies promote angiogenesis in ischemic gastrocnemius muscle. These results suggest that TUS may be more effective than SWT for the treatment of IHD and peripheral arterial disease.

Original languageEnglish (US)
Pages (from-to)2043-2049
Number of pages7
JournalCirculation Journal
Volume79
Issue number9
DOIs
StatePublished - Aug 25 2015
Externally publishedYes

Fingerprint

Peripheral Arterial Disease
Convulsive Therapy
Reperfusion
Hindlimb
Therapeutics
Acoustics
Myocardial Ischemia
Perfusion
Aptitude
Perfusion Imaging
Femoral Artery
Vascular Endothelial Growth Factor A
Skeletal Muscle
Lasers
Ischemia
Immunohistochemistry
Placebos
Technology

Keywords

  • Angiogenesis
  • Ischemic heart disease
  • Peripheral arterial disease
  • Shock wave therapy
  • Ultrasound

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Medicine(all)

Cite this

Therapeutic ultrasound promotes reperfusion and angiogenesis in a rat model of peripheral arterial disease. / Nazer, Babak; Ghahghaie, Farzin; Kashima, Risa; Khokhlova, Tatiana; Perez, Camilo; Crum, Lawrence; Matula, Tom; Hata, Akiko.

In: Circulation Journal, Vol. 79, No. 9, 25.08.2015, p. 2043-2049.

Research output: Contribution to journalArticle

Nazer, B, Ghahghaie, F, Kashima, R, Khokhlova, T, Perez, C, Crum, L, Matula, T & Hata, A 2015, 'Therapeutic ultrasound promotes reperfusion and angiogenesis in a rat model of peripheral arterial disease', Circulation Journal, vol. 79, no. 9, pp. 2043-2049. https://doi.org/10.1253/circj.CJ-15-0366
Nazer, Babak ; Ghahghaie, Farzin ; Kashima, Risa ; Khokhlova, Tatiana ; Perez, Camilo ; Crum, Lawrence ; Matula, Tom ; Hata, Akiko. / Therapeutic ultrasound promotes reperfusion and angiogenesis in a rat model of peripheral arterial disease. In: Circulation Journal. 2015 ; Vol. 79, No. 9. pp. 2043-2049.
@article{c952b7252bb347698efefa5bafbf784b,
title = "Therapeutic ultrasound promotes reperfusion and angiogenesis in a rat model of peripheral arterial disease",
abstract = "Background: Shock wave therapy (SWT) is an acoustic technology clinically used for the non-invasive treatment of ischemic heart disease (IHD). Therapeutic ultrasound (TUS) has more recently been developed for the same indication, although its effects on reperfusion and angiogenesis have yet to be directly compared to those of SWT. Methods and Results: TUS and SWT acoustic parameters were matched, and their ability to promote angiogenesis and reperfusion in a rat hindlimb ischemia model was compared. After left femoral artery excision, 3-weekly TUS, SWT or sham treatments (n=10 rats each) of the left hindlimb were performed for 2 weeks. Laser Doppler perfusion imaging demonstrated improved perfusion with TUS (66±4{\%} L:R hindlimb perfusion, mean ± SEM, P=0.02), but not with SWT (59±4{\%}, P=0.13) compared with sham (50±4{\%}). Immunohistochemistry of CD31 demonstrated increased microvascular density with TUS (222.6 vessels/high-power field, P=0.001) and SWT (216.9, P=0.01) compared to sham-treated rats (196.0). Tissue vascular endothelial growth factor mRNA levels were elevated in the left hindlimb of TUS-, but not SWT- or sham-treated rats. Conclusions: Direct comparison demonstrates that TUS is more effective than SWT at promoting reperfusion, whereas both therapies promote angiogenesis in ischemic gastrocnemius muscle. These results suggest that TUS may be more effective than SWT for the treatment of IHD and peripheral arterial disease.",
keywords = "Angiogenesis, Ischemic heart disease, Peripheral arterial disease, Shock wave therapy, Ultrasound",
author = "Babak Nazer and Farzin Ghahghaie and Risa Kashima and Tatiana Khokhlova and Camilo Perez and Lawrence Crum and Tom Matula and Akiko Hata",
year = "2015",
month = "8",
day = "25",
doi = "10.1253/circj.CJ-15-0366",
language = "English (US)",
volume = "79",
pages = "2043--2049",
journal = "Circulation Journal",
issn = "1346-9843",
publisher = "Japanese Circulation Society",
number = "9",

}

TY - JOUR

T1 - Therapeutic ultrasound promotes reperfusion and angiogenesis in a rat model of peripheral arterial disease

AU - Nazer, Babak

AU - Ghahghaie, Farzin

AU - Kashima, Risa

AU - Khokhlova, Tatiana

AU - Perez, Camilo

AU - Crum, Lawrence

AU - Matula, Tom

AU - Hata, Akiko

PY - 2015/8/25

Y1 - 2015/8/25

N2 - Background: Shock wave therapy (SWT) is an acoustic technology clinically used for the non-invasive treatment of ischemic heart disease (IHD). Therapeutic ultrasound (TUS) has more recently been developed for the same indication, although its effects on reperfusion and angiogenesis have yet to be directly compared to those of SWT. Methods and Results: TUS and SWT acoustic parameters were matched, and their ability to promote angiogenesis and reperfusion in a rat hindlimb ischemia model was compared. After left femoral artery excision, 3-weekly TUS, SWT or sham treatments (n=10 rats each) of the left hindlimb were performed for 2 weeks. Laser Doppler perfusion imaging demonstrated improved perfusion with TUS (66±4% L:R hindlimb perfusion, mean ± SEM, P=0.02), but not with SWT (59±4%, P=0.13) compared with sham (50±4%). Immunohistochemistry of CD31 demonstrated increased microvascular density with TUS (222.6 vessels/high-power field, P=0.001) and SWT (216.9, P=0.01) compared to sham-treated rats (196.0). Tissue vascular endothelial growth factor mRNA levels were elevated in the left hindlimb of TUS-, but not SWT- or sham-treated rats. Conclusions: Direct comparison demonstrates that TUS is more effective than SWT at promoting reperfusion, whereas both therapies promote angiogenesis in ischemic gastrocnemius muscle. These results suggest that TUS may be more effective than SWT for the treatment of IHD and peripheral arterial disease.

AB - Background: Shock wave therapy (SWT) is an acoustic technology clinically used for the non-invasive treatment of ischemic heart disease (IHD). Therapeutic ultrasound (TUS) has more recently been developed for the same indication, although its effects on reperfusion and angiogenesis have yet to be directly compared to those of SWT. Methods and Results: TUS and SWT acoustic parameters were matched, and their ability to promote angiogenesis and reperfusion in a rat hindlimb ischemia model was compared. After left femoral artery excision, 3-weekly TUS, SWT or sham treatments (n=10 rats each) of the left hindlimb were performed for 2 weeks. Laser Doppler perfusion imaging demonstrated improved perfusion with TUS (66±4% L:R hindlimb perfusion, mean ± SEM, P=0.02), but not with SWT (59±4%, P=0.13) compared with sham (50±4%). Immunohistochemistry of CD31 demonstrated increased microvascular density with TUS (222.6 vessels/high-power field, P=0.001) and SWT (216.9, P=0.01) compared to sham-treated rats (196.0). Tissue vascular endothelial growth factor mRNA levels were elevated in the left hindlimb of TUS-, but not SWT- or sham-treated rats. Conclusions: Direct comparison demonstrates that TUS is more effective than SWT at promoting reperfusion, whereas both therapies promote angiogenesis in ischemic gastrocnemius muscle. These results suggest that TUS may be more effective than SWT for the treatment of IHD and peripheral arterial disease.

KW - Angiogenesis

KW - Ischemic heart disease

KW - Peripheral arterial disease

KW - Shock wave therapy

KW - Ultrasound

UR - http://www.scopus.com/inward/record.url?scp=84940056058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940056058&partnerID=8YFLogxK

U2 - 10.1253/circj.CJ-15-0366

DO - 10.1253/circj.CJ-15-0366

M3 - Article

VL - 79

SP - 2043

EP - 2049

JO - Circulation Journal

JF - Circulation Journal

SN - 1346-9843

IS - 9

ER -