The ZNF217 gene amplified in breast cancers promotes immortalization of human mammary epithelial cells

Genevieve H. Nonet, Martha R. Stampfer, Kwang-Yung Chin, Joe Gray, Colin C. Collins, Paul Yaswen

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The functional consequences of overexpression of a candidate oncogene on chromosome 20q13.2, ZNF217, were examined by transducing the gene into finite life span human mammary epithelial cells (HMECs). In four independent experiments, ZNF217-transduced cultures gave rise to immortalized cells. HMECs that overcame senescence initially exhibited heterogeneous growth and continued telomere erosion, followed by increasing telomerase activity, stabilization of telomere length, and resistance to transforming growth factor β growth inhibition. The incremental changes in telomerase activity and growth that occurred in ZNF217 transduced cultures after they overcame senescence were similar to the conversion pattern we have described previously in rare HMEC lines immortalized after exposure to a chemical carcinogen. Aberrant expression of ZNF217 may be selected for during breast cancer progression because it allows breast cells to overcome senescence and attain immortality.

Original languageEnglish (US)
Pages (from-to)1250-1254
Number of pages5
JournalCancer Research
Volume61
Issue number4
StatePublished - Feb 15 2001
Externally publishedYes

Fingerprint

Breast
Epithelial Cells
Breast Neoplasms
Telomerase
Telomere
Genes
Growth
Cell Aging
Transforming Growth Factors
Oncogenes
Carcinogens
Chromosomes
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The ZNF217 gene amplified in breast cancers promotes immortalization of human mammary epithelial cells. / Nonet, Genevieve H.; Stampfer, Martha R.; Chin, Kwang-Yung; Gray, Joe; Collins, Colin C.; Yaswen, Paul.

In: Cancer Research, Vol. 61, No. 4, 15.02.2001, p. 1250-1254.

Research output: Contribution to journalArticle

Nonet, Genevieve H. ; Stampfer, Martha R. ; Chin, Kwang-Yung ; Gray, Joe ; Collins, Colin C. ; Yaswen, Paul. / The ZNF217 gene amplified in breast cancers promotes immortalization of human mammary epithelial cells. In: Cancer Research. 2001 ; Vol. 61, No. 4. pp. 1250-1254.
@article{055c1c26ea314b6cb121ccd46c3b723e,
title = "The ZNF217 gene amplified in breast cancers promotes immortalization of human mammary epithelial cells",
abstract = "The functional consequences of overexpression of a candidate oncogene on chromosome 20q13.2, ZNF217, were examined by transducing the gene into finite life span human mammary epithelial cells (HMECs). In four independent experiments, ZNF217-transduced cultures gave rise to immortalized cells. HMECs that overcame senescence initially exhibited heterogeneous growth and continued telomere erosion, followed by increasing telomerase activity, stabilization of telomere length, and resistance to transforming growth factor β growth inhibition. The incremental changes in telomerase activity and growth that occurred in ZNF217 transduced cultures after they overcame senescence were similar to the conversion pattern we have described previously in rare HMEC lines immortalized after exposure to a chemical carcinogen. Aberrant expression of ZNF217 may be selected for during breast cancer progression because it allows breast cells to overcome senescence and attain immortality.",
author = "Nonet, {Genevieve H.} and Stampfer, {Martha R.} and Kwang-Yung Chin and Joe Gray and Collins, {Colin C.} and Paul Yaswen",
year = "2001",
month = "2",
day = "15",
language = "English (US)",
volume = "61",
pages = "1250--1254",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "4",

}

TY - JOUR

T1 - The ZNF217 gene amplified in breast cancers promotes immortalization of human mammary epithelial cells

AU - Nonet, Genevieve H.

AU - Stampfer, Martha R.

AU - Chin, Kwang-Yung

AU - Gray, Joe

AU - Collins, Colin C.

AU - Yaswen, Paul

PY - 2001/2/15

Y1 - 2001/2/15

N2 - The functional consequences of overexpression of a candidate oncogene on chromosome 20q13.2, ZNF217, were examined by transducing the gene into finite life span human mammary epithelial cells (HMECs). In four independent experiments, ZNF217-transduced cultures gave rise to immortalized cells. HMECs that overcame senescence initially exhibited heterogeneous growth and continued telomere erosion, followed by increasing telomerase activity, stabilization of telomere length, and resistance to transforming growth factor β growth inhibition. The incremental changes in telomerase activity and growth that occurred in ZNF217 transduced cultures after they overcame senescence were similar to the conversion pattern we have described previously in rare HMEC lines immortalized after exposure to a chemical carcinogen. Aberrant expression of ZNF217 may be selected for during breast cancer progression because it allows breast cells to overcome senescence and attain immortality.

AB - The functional consequences of overexpression of a candidate oncogene on chromosome 20q13.2, ZNF217, were examined by transducing the gene into finite life span human mammary epithelial cells (HMECs). In four independent experiments, ZNF217-transduced cultures gave rise to immortalized cells. HMECs that overcame senescence initially exhibited heterogeneous growth and continued telomere erosion, followed by increasing telomerase activity, stabilization of telomere length, and resistance to transforming growth factor β growth inhibition. The incremental changes in telomerase activity and growth that occurred in ZNF217 transduced cultures after they overcame senescence were similar to the conversion pattern we have described previously in rare HMEC lines immortalized after exposure to a chemical carcinogen. Aberrant expression of ZNF217 may be selected for during breast cancer progression because it allows breast cells to overcome senescence and attain immortality.

UR - http://www.scopus.com/inward/record.url?scp=0035866390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035866390&partnerID=8YFLogxK

M3 - Article

VL - 61

SP - 1250

EP - 1254

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 4

ER -