The Wnt-1 proto-oncogene regulates MAP kinase activation by multiple growth factors in PC12 cells

M. G. Pan, Y. H. Wang, D. D. Hirsch, K. Labudda, Philip Stork

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

PC12/Wnt-1 cells display morphological changes in response to stimulation by select growth factors but do not respond to NGF. Furthermore, stimulation by EGF can induce neuronal differentiation in these cells but not in wild type cells. We have found that in these cells, compared to wild type PC12 cells, FGF and EGF stimulation of MAP kinase activity is enhanced, while NGF stimulation of MAP kinase in diminished. Finally, in cells expressing Wnt-1, the effect of cyclic adenosine monophosphate (cAMP) on MAP kinase activation is reversed; cAMP stimulates MAP kinase in wild type PC12 cells but inhibits MAP kinase in PC12/Wnt-1 cells. These data suggest that Wnt-1 expression alters the specificity of growth factor signaling in neuronal cells.

Original languageEnglish (US)
Pages (from-to)2005-2012
Number of pages8
JournalOncogene
Volume11
Issue number10
StatePublished - 1995

Fingerprint

Proto-Oncogenes
PC12 Cells
Intercellular Signaling Peptides and Proteins
Phosphotransferases
Nerve Growth Factor
Epidermal Growth Factor
Cyclic AMP
Cell Differentiation

Keywords

  • Growth factors
  • MAP kinase
  • PC12 cells
  • Wnt-1

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Pan, M. G., Wang, Y. H., Hirsch, D. D., Labudda, K., & Stork, P. (1995). The Wnt-1 proto-oncogene regulates MAP kinase activation by multiple growth factors in PC12 cells. Oncogene, 11(10), 2005-2012.

The Wnt-1 proto-oncogene regulates MAP kinase activation by multiple growth factors in PC12 cells. / Pan, M. G.; Wang, Y. H.; Hirsch, D. D.; Labudda, K.; Stork, Philip.

In: Oncogene, Vol. 11, No. 10, 1995, p. 2005-2012.

Research output: Contribution to journalArticle

Pan, MG, Wang, YH, Hirsch, DD, Labudda, K & Stork, P 1995, 'The Wnt-1 proto-oncogene regulates MAP kinase activation by multiple growth factors in PC12 cells', Oncogene, vol. 11, no. 10, pp. 2005-2012.
Pan, M. G. ; Wang, Y. H. ; Hirsch, D. D. ; Labudda, K. ; Stork, Philip. / The Wnt-1 proto-oncogene regulates MAP kinase activation by multiple growth factors in PC12 cells. In: Oncogene. 1995 ; Vol. 11, No. 10. pp. 2005-2012.
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