The usefulness of routine alcian blue staining of corneal specimens

J. S. Weiss, F. Orlando, Daniel Albert

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To determine the diagnostic usefulness of routine staining of corneal buttons with alcian blue, we evaluated 150 consecutive corneal specimens with hematoxylin and eosin and alcian blue stains. Overall, 67% of specimens demonstrated deposition of acid mucopolysaccharide. More specifically, positive alcian blue staining was observed in 88% of specimens with pannus, 83% of specimens with stromal downgrowth, 71% of specimens with stromal scarring, and 67% of specimens with interstitial keratitis. In 80% of these cases, the positive alcian blue stain correlated to pathology noted on hematoxylin and eosin sections. In 20% of cases, we could not explain the reason for the presence of alcian blue staining. Except for macular dystrophy of the cornea, the presence, strength, and location of staining did not provide useful information in terms of making a specific clinical diagnosis. Consequently, alcian blue should be used only in those settings in which the pathologist needs to document or confirm the abnormal presence or absence of acid mucopolysaccharide deposition.

Original languageEnglish (US)
Pages (from-to)127-132
Number of pages6
JournalCornea
Volume7
Issue number2
StatePublished - Jan 1 1988
Externally publishedYes

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Alcian Blue
Staining and Labeling
Hematoxylin
Eosine Yellowish-(YS)
Glycosaminoglycans
Coloring Agents
Acids
Keratitis
Macular Degeneration
Cornea
Cicatrix
Pathology

ASJC Scopus subject areas

  • Ophthalmology

Cite this

The usefulness of routine alcian blue staining of corneal specimens. / Weiss, J. S.; Orlando, F.; Albert, Daniel.

In: Cornea, Vol. 7, No. 2, 01.01.1988, p. 127-132.

Research output: Contribution to journalArticle

Weiss, JS, Orlando, F & Albert, D 1988, 'The usefulness of routine alcian blue staining of corneal specimens', Cornea, vol. 7, no. 2, pp. 127-132.
Weiss, J. S. ; Orlando, F. ; Albert, Daniel. / The usefulness of routine alcian blue staining of corneal specimens. In: Cornea. 1988 ; Vol. 7, No. 2. pp. 127-132.
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