The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor

Jan Tkac, James Whittaker, Tautgirdas Ruzgas

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Chitosan was chosen as a natural polymer for dispersion of single walled carbon nanotubes (SWNT) based on its ability to efficiently solubilize SWNTs to form a stable dispersion. Moreover, chitosan films deposited on a surface of a glassy carbon (GC) electrode are mechanically stable. Further stabilisation of the chitosan film containing SWNT (CHIT-SWNT) was done by chemical crosslinking with glutaraldehyde and free aldehyde groups produced a substrate used for covalent immobilisation of galactose oxidase (GalOD). Different galactose biosensor configurations were tested with optimisation of composition of inner and outer membrane; and enzyme immobilisation procedure, as well. Detection of oxygen uptake by GalOD on CHIT-SWNT layer at -400 mV is robust and, when flow injection analysis (FIA) was applied for assays, a low detection limit (25 μM) and very high assay throughput rate (150 h-1) was achieved. This new galactose biosensor offers highly reliable detection of galactose with R.S.D. well below 2% and it has been successfully applied to assaying galactose in a blood sample with recovery index between 101.2 and 102.7%.

Original languageEnglish (US)
Pages (from-to)1820-1824
Number of pages5
JournalBiosensors and Bioelectronics
Volume22
Issue number8
DOIs
StatePublished - Mar 15 2007

Fingerprint

Carbon Nanotubes
Chitosan
Biosensing Techniques
Single-walled carbon nanotubes (SWCN)
Galactose
Biosensors
Galactose Oxidase
Immobilization
Assays
Enzyme immobilization
Flow Injection Analysis
Natural polymers
Glassy carbon
Glutaral
Aldehydes
Crosslinking
Limit of Detection
Polymers
Electrodes
Blood

Keywords

  • Blood plasma
  • Carbon nanotube
  • Chitosan
  • Galactose oxidase
  • Interferents
  • Nafion

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor. / Tkac, Jan; Whittaker, James; Ruzgas, Tautgirdas.

In: Biosensors and Bioelectronics, Vol. 22, No. 8, 15.03.2007, p. 1820-1824.

Research output: Contribution to journalArticle

Tkac, Jan ; Whittaker, James ; Ruzgas, Tautgirdas. / The use of single walled carbon nanotubes dispersed in a chitosan matrix for preparation of a galactose biosensor. In: Biosensors and Bioelectronics. 2007 ; Vol. 22, No. 8. pp. 1820-1824.
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