The use of RNAi-based screens to identify host proteins involved in viral replication

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The recent development of RNAi-based techniques for protein knockdown in mammalian cells has allowed for unprecedented flexibility in the study of protein function. Currently, large siRNA libraries are available that allow the knockdown of all proteins known to be encoded by the human genome. These libraries have been used to identify the host proteins required for the replication of several clinically important viruses, including HIV, flaviviruses and influenza. This review summarizes the methods used in RNAi-based screening for host factors involved in virus replication, and discusses published examples of such screens.

Original languageEnglish (US)
Pages (from-to)303-311
Number of pages9
JournalFuture Microbiology
Volume5
Issue number2
DOIs
StatePublished - Feb 2010

Fingerprint

RNA Interference
Proteins
Flavivirus
Human Genome
Virus Replication
Human Influenza
Small Interfering RNA
Libraries
HIV
Viruses

Keywords

  • Flavivirus
  • HIV
  • Influenza
  • Screen
  • SiRNA
  • Viral host factor
  • Virus

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

The use of RNAi-based screens to identify host proteins involved in viral replication. / Hirsch, Alec.

In: Future Microbiology, Vol. 5, No. 2, 02.2010, p. 303-311.

Research output: Contribution to journalArticle

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