The use of needle biopsy for assessment of placental gene expression

Yoel Sadovsky, Solange Wyatt, Lynne Collins, Uriel Elchalal, Fredrick T. Kraus, D. Michael Nelson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: The purpose of this study was to test the hypothesis that placental samples that are obtained by needle aspiration ex vivo are useful for the determination of villus gene expression. Study design: Placental biopsy was performed with a spinal needle after uncomplicated deliveries. Villi were inspected microscopically, and RNA was extracted and analyzed with capillary electrophoresis. Gene expression was determined with quantitative polymerase chain reaction. Results: We obtained more placental villous fragments per aspiration using a 20-gauge needle (5.2 ± 1.8 fragments) than with a 22-gauge needle (3.3 ± 1.6 fragments; P <.01). RNA quality was adequate, based on the 28S and 18S recombinant RNA bands, with a mean 260/280 ratio of 1.88. The amount of extracted RNA correlated with the number of villous fragments per aspirate. Importantly, the expression of NDRG1 and hPL, both markedly altered in hypoxia, was consistent between villi that were obtained by either needle or standard biopsy. Conclusion: Placental samples that are obtained by ex vivo needle aspiration are useful for the extraction of RNA and for the determination of villous gene expression.

Original languageEnglish (US)
Pages (from-to)1137-1142
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume194
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

Needle Biopsy
Needles
Gene Expression
RNA
Biopsy
Capillary Electrophoresis
Polymerase Chain Reaction

Keywords

  • Needle aspiration
  • Placenta
  • Quantitative polymerase chain reaction
  • RNA
  • Villus

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

The use of needle biopsy for assessment of placental gene expression. / Sadovsky, Yoel; Wyatt, Solange; Collins, Lynne; Elchalal, Uriel; Kraus, Fredrick T.; Nelson, D. Michael.

In: American Journal of Obstetrics and Gynecology, Vol. 194, No. 4, 04.2006, p. 1137-1142.

Research output: Contribution to journalArticle

Sadovsky, Yoel ; Wyatt, Solange ; Collins, Lynne ; Elchalal, Uriel ; Kraus, Fredrick T. ; Nelson, D. Michael. / The use of needle biopsy for assessment of placental gene expression. In: American Journal of Obstetrics and Gynecology. 2006 ; Vol. 194, No. 4. pp. 1137-1142.
@article{0f7a9bffb82d4f8481f20464dd66abf1,
title = "The use of needle biopsy for assessment of placental gene expression",
abstract = "Objective: The purpose of this study was to test the hypothesis that placental samples that are obtained by needle aspiration ex vivo are useful for the determination of villus gene expression. Study design: Placental biopsy was performed with a spinal needle after uncomplicated deliveries. Villi were inspected microscopically, and RNA was extracted and analyzed with capillary electrophoresis. Gene expression was determined with quantitative polymerase chain reaction. Results: We obtained more placental villous fragments per aspiration using a 20-gauge needle (5.2 ± 1.8 fragments) than with a 22-gauge needle (3.3 ± 1.6 fragments; P <.01). RNA quality was adequate, based on the 28S and 18S recombinant RNA bands, with a mean 260/280 ratio of 1.88. The amount of extracted RNA correlated with the number of villous fragments per aspirate. Importantly, the expression of NDRG1 and hPL, both markedly altered in hypoxia, was consistent between villi that were obtained by either needle or standard biopsy. Conclusion: Placental samples that are obtained by ex vivo needle aspiration are useful for the extraction of RNA and for the determination of villous gene expression.",
keywords = "Needle aspiration, Placenta, Quantitative polymerase chain reaction, RNA, Villus",
author = "Yoel Sadovsky and Solange Wyatt and Lynne Collins and Uriel Elchalal and Kraus, {Fredrick T.} and Nelson, {D. Michael}",
year = "2006",
month = "4",
doi = "10.1016/j.ajog.2005.12.021",
language = "English (US)",
volume = "194",
pages = "1137--1142",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - The use of needle biopsy for assessment of placental gene expression

AU - Sadovsky, Yoel

AU - Wyatt, Solange

AU - Collins, Lynne

AU - Elchalal, Uriel

AU - Kraus, Fredrick T.

AU - Nelson, D. Michael

PY - 2006/4

Y1 - 2006/4

N2 - Objective: The purpose of this study was to test the hypothesis that placental samples that are obtained by needle aspiration ex vivo are useful for the determination of villus gene expression. Study design: Placental biopsy was performed with a spinal needle after uncomplicated deliveries. Villi were inspected microscopically, and RNA was extracted and analyzed with capillary electrophoresis. Gene expression was determined with quantitative polymerase chain reaction. Results: We obtained more placental villous fragments per aspiration using a 20-gauge needle (5.2 ± 1.8 fragments) than with a 22-gauge needle (3.3 ± 1.6 fragments; P <.01). RNA quality was adequate, based on the 28S and 18S recombinant RNA bands, with a mean 260/280 ratio of 1.88. The amount of extracted RNA correlated with the number of villous fragments per aspirate. Importantly, the expression of NDRG1 and hPL, both markedly altered in hypoxia, was consistent between villi that were obtained by either needle or standard biopsy. Conclusion: Placental samples that are obtained by ex vivo needle aspiration are useful for the extraction of RNA and for the determination of villous gene expression.

AB - Objective: The purpose of this study was to test the hypothesis that placental samples that are obtained by needle aspiration ex vivo are useful for the determination of villus gene expression. Study design: Placental biopsy was performed with a spinal needle after uncomplicated deliveries. Villi were inspected microscopically, and RNA was extracted and analyzed with capillary electrophoresis. Gene expression was determined with quantitative polymerase chain reaction. Results: We obtained more placental villous fragments per aspiration using a 20-gauge needle (5.2 ± 1.8 fragments) than with a 22-gauge needle (3.3 ± 1.6 fragments; P <.01). RNA quality was adequate, based on the 28S and 18S recombinant RNA bands, with a mean 260/280 ratio of 1.88. The amount of extracted RNA correlated with the number of villous fragments per aspirate. Importantly, the expression of NDRG1 and hPL, both markedly altered in hypoxia, was consistent between villi that were obtained by either needle or standard biopsy. Conclusion: Placental samples that are obtained by ex vivo needle aspiration are useful for the extraction of RNA and for the determination of villous gene expression.

KW - Needle aspiration

KW - Placenta

KW - Quantitative polymerase chain reaction

KW - RNA

KW - Villus

UR - http://www.scopus.com/inward/record.url?scp=33645840472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645840472&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2005.12.021

DO - 10.1016/j.ajog.2005.12.021

M3 - Article

C2 - 16580313

AN - SCOPUS:33645840472

VL - 194

SP - 1137

EP - 1142

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 4

ER -