The tyrosine kinase activity of the chicken insulin receptor is similar to that of the human insulin receptor

Hisanori Kato, Yumiko Okubo, Yuki Matsumura, Charles Roberts, Kunio Sugahara, Derek Leroith

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    The tyrosine kinase activity of a chimeric insulin receptor composed of the extracellular domain of the human insulin receptor (IR) and the intracellular domain of the chicken IR was compared with wild-type human IR. The degrees of autophosphorylation, phosphorylation of IRS-1, and in vitro phosphorylation of an exogenous substrate after stimulation by human insulin were similar to that seen with the human IR. We conclude that the insulin resistance of chickens is not attributable to a lower level of intrinsic tyrosine kinase activity of IR.

    Original languageEnglish (US)
    Pages (from-to)903-906
    Number of pages4
    JournalBioscience, Biotechnology and Biochemistry
    Volume64
    Issue number4
    StatePublished - Apr 2000

    Fingerprint

    Insulin
    Insulin Receptor
    Protein-Tyrosine Kinases
    tyrosine
    Chickens
    phosphotransferases (kinases)
    Phosphorylation
    chickens
    Insulin Resistance
    phosphorylation
    protein phosphorylation
    Substrates
    insulin resistance
    human INSR protein
    insulin receptors
    insulin

    Keywords

    • Chicken
    • Insulin receptor
    • IRS-1
    • Tyrosine kinase

    ASJC Scopus subject areas

    • Food Science
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Biotechnology
    • Chemistry (miscellaneous)
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    The tyrosine kinase activity of the chicken insulin receptor is similar to that of the human insulin receptor. / Kato, Hisanori; Okubo, Yumiko; Matsumura, Yuki; Roberts, Charles; Sugahara, Kunio; Leroith, Derek.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 4, 04.2000, p. 903-906.

    Research output: Contribution to journalArticle

    Kato, Hisanori ; Okubo, Yumiko ; Matsumura, Yuki ; Roberts, Charles ; Sugahara, Kunio ; Leroith, Derek. / The tyrosine kinase activity of the chicken insulin receptor is similar to that of the human insulin receptor. In: Bioscience, Biotechnology and Biochemistry. 2000 ; Vol. 64, No. 4. pp. 903-906.
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