The transport and turnover of phospholipids in the rat nigrostriatal system: Effects of d-amphetamine and haloperidol

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Abstract

The nigrostriatal transport of phospholipids was studied using [3H]glycerol, 32Pi and [3H]choline. [3H]glycerol was rapidly incorporated into phospholipid and significant amounts of labelled phospholipid were found in the striatum one hour after injection into the substantia nigra. In contrast, both 32Pi and [3H]choline were more slowly incorporated into phospholipid and significant amounts of labelled phospholipid were not found in the striatum until 24 hours after injection. Once incorporated into the striatum, the [3H-glycerol] phospholipids showed both a rapid (t 1/2 = 1-4 days) and a slow (t 1/2 = 14 + days) turnover component while the [32P] and [3H-Ch] phospholipids showed only a slow turnover component. The subcellular distribution of the rapidly transported [3H-glycerol] phospholipids was studied. Only [3H] phosphatidylcholine (PC) was specifically enriched in the synaptic membrane fraction. The hypothesis was tested that an increase in vesicular lysophosphatidylcholine (LPC) content is associated with dopamine (DA) release. The DA containing vesicles in the striatum were labelled by the intranigral injection of [3H] choline; seven days later, the animals were administered haloperidol to stimulate firing of the nigral-striatal DA neurons. Haloperidol significantly decreased rather than increased the [3H]LPC/[3H]PC ratio. The hypothesis was tested that chronic amphetamine treatment would inhibit phospholipid transport as a result of the decrease in neuronal activity. Chronic d-amphetamine treatment was found to have no effect on the fast component of [3H-glycerol] phospholipid turnover.

Original languageEnglish (US)
Pages (from-to)209-228
Number of pages20
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume35
Issue number2
StatePublished - 1982
Externally publishedYes

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Dextroamphetamine
Haloperidol
Rats
Phospholipids
Glycerol
Choline
Dopamine
Lysophosphatidylcholines
Substantia Nigra
Phosphatidylcholines
Injections
Corpus Striatum
Synaptic Membranes
Dopaminergic Neurons
Amphetamine
Neurons
Animals
Membranes

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

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title = "The transport and turnover of phospholipids in the rat nigrostriatal system: Effects of d-amphetamine and haloperidol",
abstract = "The nigrostriatal transport of phospholipids was studied using [3H]glycerol, 32Pi and [3H]choline. [3H]glycerol was rapidly incorporated into phospholipid and significant amounts of labelled phospholipid were found in the striatum one hour after injection into the substantia nigra. In contrast, both 32Pi and [3H]choline were more slowly incorporated into phospholipid and significant amounts of labelled phospholipid were not found in the striatum until 24 hours after injection. Once incorporated into the striatum, the [3H-glycerol] phospholipids showed both a rapid (t 1/2 = 1-4 days) and a slow (t 1/2 = 14 + days) turnover component while the [32P] and [3H-Ch] phospholipids showed only a slow turnover component. The subcellular distribution of the rapidly transported [3H-glycerol] phospholipids was studied. Only [3H] phosphatidylcholine (PC) was specifically enriched in the synaptic membrane fraction. The hypothesis was tested that an increase in vesicular lysophosphatidylcholine (LPC) content is associated with dopamine (DA) release. The DA containing vesicles in the striatum were labelled by the intranigral injection of [3H] choline; seven days later, the animals were administered haloperidol to stimulate firing of the nigral-striatal DA neurons. Haloperidol significantly decreased rather than increased the [3H]LPC/[3H]PC ratio. The hypothesis was tested that chronic amphetamine treatment would inhibit phospholipid transport as a result of the decrease in neuronal activity. Chronic d-amphetamine treatment was found to have no effect on the fast component of [3H-glycerol] phospholipid turnover.",
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T1 - The transport and turnover of phospholipids in the rat nigrostriatal system

T2 - Effects of d-amphetamine and haloperidol

AU - Hitzemann, Robert

AU - Loh, H.

PY - 1982

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N2 - The nigrostriatal transport of phospholipids was studied using [3H]glycerol, 32Pi and [3H]choline. [3H]glycerol was rapidly incorporated into phospholipid and significant amounts of labelled phospholipid were found in the striatum one hour after injection into the substantia nigra. In contrast, both 32Pi and [3H]choline were more slowly incorporated into phospholipid and significant amounts of labelled phospholipid were not found in the striatum until 24 hours after injection. Once incorporated into the striatum, the [3H-glycerol] phospholipids showed both a rapid (t 1/2 = 1-4 days) and a slow (t 1/2 = 14 + days) turnover component while the [32P] and [3H-Ch] phospholipids showed only a slow turnover component. The subcellular distribution of the rapidly transported [3H-glycerol] phospholipids was studied. Only [3H] phosphatidylcholine (PC) was specifically enriched in the synaptic membrane fraction. The hypothesis was tested that an increase in vesicular lysophosphatidylcholine (LPC) content is associated with dopamine (DA) release. The DA containing vesicles in the striatum were labelled by the intranigral injection of [3H] choline; seven days later, the animals were administered haloperidol to stimulate firing of the nigral-striatal DA neurons. Haloperidol significantly decreased rather than increased the [3H]LPC/[3H]PC ratio. The hypothesis was tested that chronic amphetamine treatment would inhibit phospholipid transport as a result of the decrease in neuronal activity. Chronic d-amphetamine treatment was found to have no effect on the fast component of [3H-glycerol] phospholipid turnover.

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