The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome

Masataka Kasai, Takao Matsuzaki, Katsuo Katayanagi, Akira Omori, Richard Maziarz, Jack L. Strominger, Katsunori Aoki, Kenji Suzuki

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

We previously showed that consensus sequences exist at the chromosomal breakpoints in lymphoid malignancies and that these sequences are specifically recognized by a novel DNA binding protein, Translin. In the present study, the native form of Translin was established to be a ring- shaped structure by electron microscopy and crystallographic studies. It was also determined that this multimeric Translin formed by the subunits is responsible for its binding to target sequences situated only at single- stranded DNA ends. Furthermore, DNA-damaging reagents were found to initiate a signaling pathway for the active nuclear transport of Translin. The results support the hypothesis that staggered breaks occur at recombination hot spots and Translin has a pivotal function in recognition of the generated single- stranded DNA ends.

Original languageEnglish (US)
Pages (from-to)11402-11407
Number of pages6
JournalJournal of Biological Chemistry
Volume272
Issue number17
DOIs
StatePublished - Apr 25 1997

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Single-Stranded DNA
Human Genome
Genetic Recombination
Genes
Cell Nucleus Active Transport
Active Biological Transport
DNA
Consensus Sequence
DNA-Binding Proteins
Electron microscopy
Electron Microscopy
Neoplasms

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kasai, M., Matsuzaki, T., Katayanagi, K., Omori, A., Maziarz, R., Strominger, J. L., ... Suzuki, K. (1997). The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome. Journal of Biological Chemistry, 272(17), 11402-11407. https://doi.org/10.1074/jbc.272.17.11402

The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome. / Kasai, Masataka; Matsuzaki, Takao; Katayanagi, Katsuo; Omori, Akira; Maziarz, Richard; Strominger, Jack L.; Aoki, Katsunori; Suzuki, Kenji.

In: Journal of Biological Chemistry, Vol. 272, No. 17, 25.04.1997, p. 11402-11407.

Research output: Contribution to journalArticle

Kasai, M, Matsuzaki, T, Katayanagi, K, Omori, A, Maziarz, R, Strominger, JL, Aoki, K & Suzuki, K 1997, 'The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome', Journal of Biological Chemistry, vol. 272, no. 17, pp. 11402-11407. https://doi.org/10.1074/jbc.272.17.11402
Kasai, Masataka ; Matsuzaki, Takao ; Katayanagi, Katsuo ; Omori, Akira ; Maziarz, Richard ; Strominger, Jack L. ; Aoki, Katsunori ; Suzuki, Kenji. / The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 17. pp. 11402-11407.
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