The T cell reactivity against the major merozoite protein of Plasmodium falciparum

Andrea Crisanti, Klaus Frueh, Hans Michael Müller, Hermann Bujard

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have undertaken a systematic search for T cell epitopes within the sequence of the major merozoite surface antigen (GP190) of Plasmodium falciparum. Recombinant polypeptides expressed in E. coli were used to evaluate the reactivity of peripheral blood monuclear cells (PBMC) from both inhabitants of a rural community of West Africa exposed to P. falciparum transmission and from German patients with diagnosis of acute malaria. Although the proliferative response of the PBMC was in most cases very low, several T cell clones could be established. Deletion analysis of each gp190-derived polypeptide allowed the identification of six different T cell epitopes. Epitopes could be mapped within the dimorphic region of gp190, which also contains the sequences most frequently recognized by sera from adult individuals living in endemic areas.

Original languageEnglish (US)
Pages (from-to)143-148
Number of pages6
JournalImmunology Letters
Volume25
Issue number1-3
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Merozoites
T-Lymphocyte Epitopes
Plasmodium falciparum
Blood Cells
T-Lymphocytes
Peptides
Western Africa
Rural Population
Surface Antigens
Epitopes
Proteins
Clone Cells
Escherichia coli
Serum
Acute malaria

Keywords

  • Dimorphic sequence
  • Plasmodium falciparum gp190
  • T cell epitopes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

The T cell reactivity against the major merozoite protein of Plasmodium falciparum. / Crisanti, Andrea; Frueh, Klaus; Müller, Hans Michael; Bujard, Hermann.

In: Immunology Letters, Vol. 25, No. 1-3, 1990, p. 143-148.

Research output: Contribution to journalArticle

Crisanti, Andrea ; Frueh, Klaus ; Müller, Hans Michael ; Bujard, Hermann. / The T cell reactivity against the major merozoite protein of Plasmodium falciparum. In: Immunology Letters. 1990 ; Vol. 25, No. 1-3. pp. 143-148.
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