The T cell reactivity against the major merozoite protein of Plasmodium falciparum

Andrea Crisanti, Klaus Frueh, Hans Michael Müller, Hermann Bujard

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8 Scopus citations

Abstract

We have undertaken a systematic search for T cell epitopes within the sequence of the major merozoite surface antigen (GP190) of Plasmodium falciparum. Recombinant polypeptides expressed in E. coli were used to evaluate the reactivity of peripheral blood monuclear cells (PBMC) from both inhabitants of a rural community of West Africa exposed to P. falciparum transmission and from German patients with diagnosis of acute malaria. Although the proliferative response of the PBMC was in most cases very low, several T cell clones could be established. Deletion analysis of each gp190-derived polypeptide allowed the identification of six different T cell epitopes. Epitopes could be mapped within the dimorphic region of gp190, which also contains the sequences most frequently recognized by sera from adult individuals living in endemic areas.

Original languageEnglish (US)
Pages (from-to)143-148
Number of pages6
JournalImmunology Letters
Volume25
Issue number1-3
DOIs
Publication statusPublished - 1990
Externally publishedYes

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Keywords

  • Dimorphic sequence
  • Plasmodium falciparum gp190
  • T cell epitopes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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