The synaptic cell adhesion molecule, SynCAM1, mediates astrocyte-to- astrocyte and astrocyte-to-GnRH neuron adhesiveness in the mouse hypothalamus

Ursula S. Sandau, Alison E. Mungenast, Jack McCarthy, Thomas Biederer, Gabriel Corfas, Sergio Ojeda

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    We previously identified synaptic cell adhesion molecule 1 (SynCAM1) as a component of a genetic network involved in the hypothalamic control of female puberty. Although it is well established that SynCAM1 is a synaptic adhesion molecule, its contribution to hypothalamic function is unknown. Here we show that, in addition to the expected neuronal localization illustrated by its presence in GnRH neurons, SynCAM1 is expressed in hypothalamic astrocytes. Cell adhesion assays indicated that SynCAM is recognized by both GnRH neurons and astrocytes as an adhesive partner and promotes cell-cell adhesiveness via homophilic, extracellular domain-mediated interactions. Alternative splicing of the SynCAM1 primary mRNA transcript yields four mRNAs encoding membrane-spanning SynCAM1 isoforms. Variants 1 and 4 are predicted to be both N and O glycosylated. Hypothalamic astrocytes and GnRH-producing GT1-7 cells express mainly isoform 4 mRNA, and sequential N- and O-deglycosylation of proteins extracted from these cells yields progressively smaller SynCAM1 species, indicating that isoform 4 is the predominant SynCAM1 variant expressed in astrocytes and GT1-7 cells. Neither cell type expresses the products of two other SynCAM genes (SynCAM2 and SynCAM3), suggesting that SynCAM-mediated astrocyte-astrocyte and astrocyte-GnRH neuron adhesiveness is mostly mediated by SynCAM1 homophilic interactions. When erbB4 receptor function is disrupted in astrocytes, via transgenic expression of a dominant-negative erbB4 receptor form, SynCAM1-mediated adhesiveness is severely compromised. Conversely, SynCAM1 adhesive behavior is rapidly, but transiently, enhanced in astrocytes by ligand-dependent activation of erbB4 receptors, suggesting that erbB4-mediated events affecting SynCAM1 function contribute to regulate astrocyte adhesive communication.

    Original languageEnglish (US)
    Pages (from-to)2353-2363
    Number of pages11
    JournalEndocrinology
    Volume152
    Issue number6
    DOIs
    StatePublished - Jun 2011

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    Adhesiveness
    Cell Adhesion Molecules
    Gonadotropin-Releasing Hormone
    Astrocytes
    Hypothalamus
    Neurons
    Adhesives
    Protein Isoforms
    RNA Isoforms
    Synaptic Membranes
    Messenger RNA
    Neurotransmitter Receptor
    Alternative Splicing
    Puberty
    Cell Adhesion
    Communication

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    The synaptic cell adhesion molecule, SynCAM1, mediates astrocyte-to- astrocyte and astrocyte-to-GnRH neuron adhesiveness in the mouse hypothalamus. / Sandau, Ursula S.; Mungenast, Alison E.; McCarthy, Jack; Biederer, Thomas; Corfas, Gabriel; Ojeda, Sergio.

    In: Endocrinology, Vol. 152, No. 6, 06.2011, p. 2353-2363.

    Research output: Contribution to journalArticle

    Sandau, Ursula S. ; Mungenast, Alison E. ; McCarthy, Jack ; Biederer, Thomas ; Corfas, Gabriel ; Ojeda, Sergio. / The synaptic cell adhesion molecule, SynCAM1, mediates astrocyte-to- astrocyte and astrocyte-to-GnRH neuron adhesiveness in the mouse hypothalamus. In: Endocrinology. 2011 ; Vol. 152, No. 6. pp. 2353-2363.
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