Abstract
Aims: The diagnosis of prostate carcinoma is based on a constellation of architectural, nuclear, cytoplasmic, and ancillary features. The aim of this study was to determine if the Ventana Symphony H&E protocol can improve on the detection of prominent nucleoli in foci of prostate cancer. Methods: One hundred and twenty cases of Gleason score 3 + 3 = 6 cancers involving 10-30% of one core were retrieved from four academic institutions and two large laboratories (20 cases per institution). The routine H&E and Symphony™ H&E protocol stained slides from the same case were reviewed centrally in a blinded fashion at the Johns Hopkins Hospital. The mean percentages of prominent nucleoli in malignant and benign glands by visual estimation were recorded for each case. After unblinding, the routine H&E slides were compared in a pair-wise t-test with the corresponding Symphony™ stained slide. Results: Within all sites, the Symphony™ slides showed statistically significantly more prominent nucleoli in the malignant glands than the routine slides. Conclusions: The Symphony™ H&E staining protocol consistently highlights nucleoli in cancer to a greater extent than routine H&E stains and may increase the likelihood of making a diagnosis of limited adenocarcinoma of the prostate in challenging cases.
Original language | English (US) |
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Pages (from-to) | 450-456 |
Number of pages | 7 |
Journal | Pathology |
Volume | 40 |
Issue number | 5 |
DOIs | |
State | Published - Aug 2008 |
Externally published | Yes |
Keywords
- Cancer diagnosis
- Nucleoli
- Prostate cancer
- Symphony staining protocol
ASJC Scopus subject areas
- Pathology and Forensic Medicine