The subcellular distribution of alpha-tocopherol in the adult primate brain and its relationship with membrane arachidonic acid and its oxidation products

Emily S. Mohn, Matthew J. Kuchan, John W. Erdman, Martha Neuringer, Nirupa R. Matthan, Chung Yen Oliver Chen, Elizabeth J. Johnson

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The relationship between α-tocopherol, a known antioxidant, and polyunsaturated fatty acid (PUFA) oxidation, has not been directly investigated in the primate brain. This study characterized the membrane distribution of α-tocopherol in brain regions and investigated the association between membrane α-tocopherol and PUFA content, as well as brain PUFA oxidation products. Nuclear, myelin, mitochondrial, and neuronal membranes were isolated using a density gradient from the prefrontal cortex (PFC), cerebellum (CER), striatum (ST), and hippocampus (HC) of adult rhesus monkeys (n = 9), fed a stock diet containing vitamin E (α -γ-tocopherol intake: ~0.7 µmol/kg body weight/day, ~5 µmol/kg body weight/day, respectively). α -tocopherol, PUFAs, and PUFA oxidation products were measured using high performance liquid chromatography (HPLC), gas chromatography (GC) and liquid chromatography-gas chromatography/mass spectrometry (LC-GC/MS) respectively. α-Tocopherol (ng/mg protein) was highest in nuclear membranes (p < 0.05) for all regions except HC. In PFC and ST, arachidonic acid (AA, µg/mg protein) had a similar membrane distribution to α-tocopherol. Total α-tocopherol concentrations were inversely associated with AA oxidation products (isoprostanes) (p < 0.05), but not docosahexaenoic acid oxidation products (neuroprostanes). This study reports novel data on α-tocopherol accumulation in primate brain regions and membranes and provides evidence that α-tocopherol and AA are similarly distributed in PFC and ST membranes, which may reflect a protective effect of α-tocopherol against AA oxidation.

    Original languageEnglish (US)
    Article number97
    JournalAntioxidants
    Volume6
    Issue number4
    DOIs
    StatePublished - Dec 1 2017

    Fingerprint

    Tocopherols
    alpha-Tocopherol
    Arachidonic Acid
    Primates
    Brain
    Membranes
    Oxidation
    Unsaturated Fatty Acids
    Prefrontal Cortex
    Gas chromatography
    Neuroprostanes
    Hippocampus
    Body Weight
    Isoprostanes
    Docosahexaenoic Acids
    Liquid chromatography
    Nuclear Envelope
    High performance liquid chromatography
    Mitochondrial Membranes
    Nutrition

    Keywords

    • Arachidonic acid
    • Brain
    • Isoprostanes
    • Membranes
    • Rhesus monkey
    • α-tocopherol

    ASJC Scopus subject areas

    • Physiology
    • Biochemistry
    • Molecular Biology
    • Clinical Biochemistry
    • Cell Biology

    Cite this

    The subcellular distribution of alpha-tocopherol in the adult primate brain and its relationship with membrane arachidonic acid and its oxidation products. / Mohn, Emily S.; Kuchan, Matthew J.; Erdman, John W.; Neuringer, Martha; Matthan, Nirupa R.; Oliver Chen, Chung Yen; Johnson, Elizabeth J.

    In: Antioxidants, Vol. 6, No. 4, 97, 01.12.2017.

    Research output: Contribution to journalArticle

    Mohn, Emily S. ; Kuchan, Matthew J. ; Erdman, John W. ; Neuringer, Martha ; Matthan, Nirupa R. ; Oliver Chen, Chung Yen ; Johnson, Elizabeth J. / The subcellular distribution of alpha-tocopherol in the adult primate brain and its relationship with membrane arachidonic acid and its oxidation products. In: Antioxidants. 2017 ; Vol. 6, No. 4.
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    AU - Oliver Chen, Chung Yen

    AU - Johnson, Elizabeth J.

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