The structure of lombricine kinase: Implications for phosphagen kinase conformational changes

D. Jeffrey Bush, Olga Kirillova, Shawn A. Clark, Omar Davulcu, Felcy Fabiola, Qing Xie, Thayumanasamy Somasundaram, W. Ross Ellington, Michael S. Chapman

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Lombricine kinase is a member of the phosphagen kinase family and a homolog of creatine and arginine kinases, enzymes responsible for buffering cellular ATP levels. Structures of lombricine kinase from the marine worm Urechis caupo were determined by x-ray crystallography. One form was crystallized as a nucleotide complex, and the other was substrate-free. The two structures are similar to each other and more similar to the substrate-free forms of homologs than to the substrate-bound forms of the other phosphagen kinases. Active site specificity loop 309-317, which is disordered in substrate-free structures of homologs and is known from the NMR of arginine kinase to be inherently dynamic, is resolved in both lombricine kinase structures, providing an improved basis for understanding the loop dynamics. Phosphagen kinases undergo a segmented closing on substrate binding, but the lombricine kinase ADP complex is in the open form more typical of substrate-free homologs. Through a comparison with prior complexes of intermediate structure, a correlation was revealed between the overall enzyme conformation and the substrate interactions of His 178. Comparative modeling provides a rationale for the more relaxed specificity of these kinases, of which the natural substrates are among the largest of the phosphagen substrates.

Original languageEnglish (US)
Pages (from-to)9338-9350
Number of pages13
JournalJournal of Biological Chemistry
Volume286
Issue number11
DOIs
StatePublished - Mar 18 2011

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Bush, D. J., Kirillova, O., Clark, S. A., Davulcu, O., Fabiola, F., Xie, Q., Somasundaram, T., Ellington, W. R., & Chapman, M. S. (2011). The structure of lombricine kinase: Implications for phosphagen kinase conformational changes. Journal of Biological Chemistry, 286(11), 9338-9350. https://doi.org/10.1074/jbc.M110.202796