TY - JOUR
T1 - The structure of lombricine kinase
T2 - Implications for phosphagen kinase conformational changes
AU - Bush, D. Jeffrey
AU - Kirillova, Olga
AU - Clark, Shawn A.
AU - Davulcu, Omar
AU - Fabiola, Felcy
AU - Xie, Qing
AU - Somasundaram, Thayumanasamy
AU - Ellington, W. Ross
AU - Chapman, Michael S.
PY - 2011/3/18
Y1 - 2011/3/18
N2 - Lombricine kinase is a member of the phosphagen kinase family and a homolog of creatine and arginine kinases, enzymes responsible for buffering cellular ATP levels. Structures of lombricine kinase from the marine worm Urechis caupo were determined by x-ray crystallography. One form was crystallized as a nucleotide complex, and the other was substrate-free. The two structures are similar to each other and more similar to the substrate-free forms of homologs than to the substrate-bound forms of the other phosphagen kinases. Active site specificity loop 309-317, which is disordered in substrate-free structures of homologs and is known from the NMR of arginine kinase to be inherently dynamic, is resolved in both lombricine kinase structures, providing an improved basis for understanding the loop dynamics. Phosphagen kinases undergo a segmented closing on substrate binding, but the lombricine kinase ADP complex is in the open form more typical of substrate-free homologs. Through a comparison with prior complexes of intermediate structure, a correlation was revealed between the overall enzyme conformation and the substrate interactions of His 178. Comparative modeling provides a rationale for the more relaxed specificity of these kinases, of which the natural substrates are among the largest of the phosphagen substrates.
AB - Lombricine kinase is a member of the phosphagen kinase family and a homolog of creatine and arginine kinases, enzymes responsible for buffering cellular ATP levels. Structures of lombricine kinase from the marine worm Urechis caupo were determined by x-ray crystallography. One form was crystallized as a nucleotide complex, and the other was substrate-free. The two structures are similar to each other and more similar to the substrate-free forms of homologs than to the substrate-bound forms of the other phosphagen kinases. Active site specificity loop 309-317, which is disordered in substrate-free structures of homologs and is known from the NMR of arginine kinase to be inherently dynamic, is resolved in both lombricine kinase structures, providing an improved basis for understanding the loop dynamics. Phosphagen kinases undergo a segmented closing on substrate binding, but the lombricine kinase ADP complex is in the open form more typical of substrate-free homologs. Through a comparison with prior complexes of intermediate structure, a correlation was revealed between the overall enzyme conformation and the substrate interactions of His 178. Comparative modeling provides a rationale for the more relaxed specificity of these kinases, of which the natural substrates are among the largest of the phosphagen substrates.
UR - http://www.scopus.com/inward/record.url?scp=79953187922&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953187922&partnerID=8YFLogxK
U2 - 10.1074/jbc.M110.202796
DO - 10.1074/jbc.M110.202796
M3 - Article
C2 - 21212263
AN - SCOPUS:79953187922
SN - 0021-9258
VL - 286
SP - 9338
EP - 9350
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 11
ER -