The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold

Debanu Das, Piotr Kozbial, Gye Won Han, Dennis Carlton, Lukasz Jaroszewski, Polat Abdubek, Tamara Astakhova, Herbert L. Axelrod, Constantina Bakolitsa, Connie Chen, Hsiu Ju Chiu, Michelle Chiu, Thomas Clayton, Marc C. Deller, Lian Duan, Kyle Ellrott, Marc André Elsliger, Dustin Ernst, Carol L. Farr, Julie Feuerhelm & 33 others Anna Grzechnik, Joanna C. Grant, Kevin K. Jin, Hope A. Johnson, Heath E. Klock, Mark W. Knuth, S. Sri Krishna, Abhinav Kumar, David Marciano, Daniel McMullan, Mitchell D. Miller, Andrew T. Morse, Edward Nigoghossian, Amanda Nopakun, Linda Okach, Silvya Oommachen, Jessica Paulsen, Christina Puckett, Ron Reyes, Christopher L. Rife, Natasha Sefcovic, Henry J. Tien, Christine B. Trame, Henry Van Den Bedem, Dana Weekes, Tiffany Wooten, Qingping Xu, Keith O. Hodgson, John Wooley, Ashley M. Deacon, Adam Godzik, Scott A. Lesley, Ian A. Wilson

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

KPN03535 (gi|152972051) is a putative lipoprotein of unknown function that is secreted by Klebsiella pneumoniae MGH 78578. The crystal structure reveals that despite a lack of any detectable sequence similarity to known structures, it is a novel variant of the OB-fold and structurally similar to the bacterial Cpx-pathway protein NlpE, single-stranded DNA-binding (SSB) proteins and toxins. K. pneumoniae MGH 78578 forms part of the normal human skin, mouth and gut flora and is an opportunistic pathogen that is linked to about 8% of all hospital-acquired infections in the USA. This structure provides the foundation for further investigations into this divergent member of the OB-fold family.

Original languageEnglish (US)
Pages (from-to)1254-1260
Number of pages7
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number10
DOIs
StatePublished - Oct 2010
Externally publishedYes

Fingerprint

Klebsiella
pneumonia
lipoproteins
DNA-Binding Proteins
Klebsiella pneumoniae
Pathogens
Lipoproteins
Skin
Crystal structure
plants (botany)
proteins
pathogens
mouth
infectious diseases
Cross Infection
Mouth
Proteins
deoxyribonucleic acid
crystal structure

Keywords

  • BOF
  • human gut pathogens
  • lipoproteins
  • NipE-like protein
  • OB-fold
  • single-stranded DNA-binding proteins
  • structural genomics
  • toxins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold. / Das, Debanu; Kozbial, Piotr; Han, Gye Won; Carlton, Dennis; Jaroszewski, Lukasz; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Bakolitsa, Constantina; Chen, Connie; Chiu, Hsiu Ju; Chiu, Michelle; Clayton, Thomas; Deller, Marc C.; Duan, Lian; Ellrott, Kyle; Elsliger, Marc André; Ernst, Dustin; Farr, Carol L.; Feuerhelm, Julie; Grzechnik, Anna; Grant, Joanna C.; Jin, Kevin K.; Johnson, Hope A.; Klock, Heath E.; Knuth, Mark W.; Krishna, S. Sri; Kumar, Abhinav; Marciano, David; McMullan, Daniel; Miller, Mitchell D.; Morse, Andrew T.; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Oommachen, Silvya; Paulsen, Jessica; Puckett, Christina; Reyes, Ron; Rife, Christopher L.; Sefcovic, Natasha; Tien, Henry J.; Trame, Christine B.; Van Den Bedem, Henry; Weekes, Dana; Wooten, Tiffany; Xu, Qingping; Hodgson, Keith O.; Wooley, John; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 10, 10.2010, p. 1254-1260.

Research output: Contribution to journalArticle

Das, D, Kozbial, P, Han, GW, Carlton, D, Jaroszewski, L, Abdubek, P, Astakhova, T, Axelrod, HL, Bakolitsa, C, Chen, C, Chiu, HJ, Chiu, M, Clayton, T, Deller, MC, Duan, L, Ellrott, K, Elsliger, MA, Ernst, D, Farr, CL, Feuerhelm, J, Grzechnik, A, Grant, JC, Jin, KK, Johnson, HA, Klock, HE, Knuth, MW, Krishna, SS, Kumar, A, Marciano, D, McMullan, D, Miller, MD, Morse, AT, Nigoghossian, E, Nopakun, A, Okach, L, Oommachen, S, Paulsen, J, Puckett, C, Reyes, R, Rife, CL, Sefcovic, N, Tien, HJ, Trame, CB, Van Den Bedem, H, Weekes, D, Wooten, T, Xu, Q, Hodgson, KO, Wooley, J, Deacon, AM, Godzik, A, Lesley, SA & Wilson, IA 2010, 'The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 66, no. 10, pp. 1254-1260. https://doi.org/10.1107/S1744309109018168
Das, Debanu ; Kozbial, Piotr ; Han, Gye Won ; Carlton, Dennis ; Jaroszewski, Lukasz ; Abdubek, Polat ; Astakhova, Tamara ; Axelrod, Herbert L. ; Bakolitsa, Constantina ; Chen, Connie ; Chiu, Hsiu Ju ; Chiu, Michelle ; Clayton, Thomas ; Deller, Marc C. ; Duan, Lian ; Ellrott, Kyle ; Elsliger, Marc André ; Ernst, Dustin ; Farr, Carol L. ; Feuerhelm, Julie ; Grzechnik, Anna ; Grant, Joanna C. ; Jin, Kevin K. ; Johnson, Hope A. ; Klock, Heath E. ; Knuth, Mark W. ; Krishna, S. Sri ; Kumar, Abhinav ; Marciano, David ; McMullan, Daniel ; Miller, Mitchell D. ; Morse, Andrew T. ; Nigoghossian, Edward ; Nopakun, Amanda ; Okach, Linda ; Oommachen, Silvya ; Paulsen, Jessica ; Puckett, Christina ; Reyes, Ron ; Rife, Christopher L. ; Sefcovic, Natasha ; Tien, Henry J. ; Trame, Christine B. ; Van Den Bedem, Henry ; Weekes, Dana ; Wooten, Tiffany ; Xu, Qingping ; Hodgson, Keith O. ; Wooley, John ; Deacon, Ashley M. ; Godzik, Adam ; Lesley, Scott A. ; Wilson, Ian A. / The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2010 ; Vol. 66, No. 10. pp. 1254-1260.
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AU - Abdubek, Polat

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AU - Krishna, S. Sri

AU - Kumar, Abhinav

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AU - Xu, Qingping

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