The Streptococcus mutans irvA Gene Encodes a trans-Acting Riboregulatory mRNA

Nan Liu, Guoqing Niu, Zhoujie Xie, Zhiyun Chen, Andreas Itzek, Jens Kreth, Allison Gillaspy, Lin Zeng, Robert Burne, Fengxia Qi, Justin Merritt

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In both prokaryotes and eukaryotes, insight into gene function is typically obtained by insilico homology searches and/or phenotypic analyses of strains bearing mutations within open reading frames. However, the studies herein illustrate how mRNA function is not limited to the expression of a cognate protein. We demonstrate that a stress-induced protein-encoding mRNA (irvA) from the dental caries pathogen Streptococcus mutans directly modulates target mRNA (gbpC) stability through seed pairing interactions. The 5' untranslated region of irvA mRNA isa trans riboregulator of gbpC and a critical activator of the DDAG stress response, whereas IrvA functions independently in the regulation of natural competence. The irvA riboregulatory domain controls GbpC production by forming irvA-gbpC hybrid mRNA duplexes that prevent gbpC degradation byan RNase J2-mediated pathway. These studies implicate a potentially ubiquitous role for typical protein-encoding mRNAs as riboregulators, which could alter current concepts in gene regulation.

Original languageEnglish (US)
Pages (from-to)179-190
Number of pages12
JournalMolecular Cell
Volume57
Issue number1
DOIs
StatePublished - Jan 8 2015
Externally publishedYes

Fingerprint

Streptococcus mutans
Messenger RNA
Genes
5' Untranslated Regions
RNA Stability
Dental Caries
Ribonucleases
Heat-Shock Proteins
Eukaryota
Mental Competency
Open Reading Frames
Seeds
Proteins
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The Streptococcus mutans irvA Gene Encodes a trans-Acting Riboregulatory mRNA. / Liu, Nan; Niu, Guoqing; Xie, Zhoujie; Chen, Zhiyun; Itzek, Andreas; Kreth, Jens; Gillaspy, Allison; Zeng, Lin; Burne, Robert; Qi, Fengxia; Merritt, Justin.

In: Molecular Cell, Vol. 57, No. 1, 08.01.2015, p. 179-190.

Research output: Contribution to journalArticle

Liu, N, Niu, G, Xie, Z, Chen, Z, Itzek, A, Kreth, J, Gillaspy, A, Zeng, L, Burne, R, Qi, F & Merritt, J 2015, 'The Streptococcus mutans irvA Gene Encodes a trans-Acting Riboregulatory mRNA', Molecular Cell, vol. 57, no. 1, pp. 179-190. https://doi.org/10.1016/j.molcel.2014.11.003
Liu, Nan ; Niu, Guoqing ; Xie, Zhoujie ; Chen, Zhiyun ; Itzek, Andreas ; Kreth, Jens ; Gillaspy, Allison ; Zeng, Lin ; Burne, Robert ; Qi, Fengxia ; Merritt, Justin. / The Streptococcus mutans irvA Gene Encodes a trans-Acting Riboregulatory mRNA. In: Molecular Cell. 2015 ; Vol. 57, No. 1. pp. 179-190.
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