The specificity of the response of preoptic-septal area neurons to estrogen: 17α-estradiol versus 17β-estradiol and the response of extrahypothalamic neurons

Martin Kelly, R. L. Moss, C. A. Dudley, C. P. Fawcett

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

The purpose of this study was to determine the specificity of the response of medial preoptic-septal neurons (mPOA-S) to microelectrophoresed 17β-estradiol hemisuccinate (17βE2S). In vitro studies were conducted initially to determine the release of the labeled 17βE2S from multibarrel glass micropipettes. Subsequently, an isomer of 17βE2S, 17α-estradiol hemisuccinate (17αE2S), was synthesized and purified. Thirty-six mPOA-S neurons from normal cycling female rats were tested with both 17βE2S and 17αE2S. Twelve of these units responded with inhibition to 17βE2S, while none responded to 17αE2S. Furthermore, fifty extrahypothalamic (cortical, hippocampal, thalamic) neurons were tested with 17βE2S. The majority (N = 45) showed no response, three showed excitation and two inhibition to the microelectrophoresed steroid ester. These findings suggest that a specific receptor mechanism is responsible for the changes in mPOA-S unit activity, and that these effects may be important in the regulation of reproductive events.

Original languageEnglish (US)
Pages (from-to)43-52
Number of pages10
JournalExperimental Brain Research
Volume30
Issue number1
DOIs
StatePublished - Oct 1977
Externally publishedYes

Fingerprint

Septum of Brain
Preoptic Area
Estradiol
Estrogens
Neurons
Glass
Esters
Steroids

Keywords

  • Estradiol
  • Extrahypothalamic neurons
  • Preoptic area
  • Stereospecificity
  • Transport number

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The specificity of the response of preoptic-septal area neurons to estrogen : 17α-estradiol versus 17β-estradiol and the response of extrahypothalamic neurons. / Kelly, Martin; Moss, R. L.; Dudley, C. A.; Fawcett, C. P.

In: Experimental Brain Research, Vol. 30, No. 1, 10.1977, p. 43-52.

Research output: Contribution to journalArticle

@article{52916e8d39d641e3b84fca644cb85369,
title = "The specificity of the response of preoptic-septal area neurons to estrogen: 17α-estradiol versus 17β-estradiol and the response of extrahypothalamic neurons",
abstract = "The purpose of this study was to determine the specificity of the response of medial preoptic-septal neurons (mPOA-S) to microelectrophoresed 17β-estradiol hemisuccinate (17βE2S). In vitro studies were conducted initially to determine the release of the labeled 17βE2S from multibarrel glass micropipettes. Subsequently, an isomer of 17βE2S, 17α-estradiol hemisuccinate (17αE2S), was synthesized and purified. Thirty-six mPOA-S neurons from normal cycling female rats were tested with both 17βE2S and 17αE2S. Twelve of these units responded with inhibition to 17βE2S, while none responded to 17αE2S. Furthermore, fifty extrahypothalamic (cortical, hippocampal, thalamic) neurons were tested with 17βE2S. The majority (N = 45) showed no response, three showed excitation and two inhibition to the microelectrophoresed steroid ester. These findings suggest that a specific receptor mechanism is responsible for the changes in mPOA-S unit activity, and that these effects may be important in the regulation of reproductive events.",
keywords = "Estradiol, Extrahypothalamic neurons, Preoptic area, Stereospecificity, Transport number",
author = "Martin Kelly and Moss, {R. L.} and Dudley, {C. A.} and Fawcett, {C. P.}",
year = "1977",
month = "10",
doi = "10.1007/BF00237857",
language = "English (US)",
volume = "30",
pages = "43--52",
journal = "Experimental Brain Research",
issn = "0014-4819",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - The specificity of the response of preoptic-septal area neurons to estrogen

T2 - 17α-estradiol versus 17β-estradiol and the response of extrahypothalamic neurons

AU - Kelly, Martin

AU - Moss, R. L.

AU - Dudley, C. A.

AU - Fawcett, C. P.

PY - 1977/10

Y1 - 1977/10

N2 - The purpose of this study was to determine the specificity of the response of medial preoptic-septal neurons (mPOA-S) to microelectrophoresed 17β-estradiol hemisuccinate (17βE2S). In vitro studies were conducted initially to determine the release of the labeled 17βE2S from multibarrel glass micropipettes. Subsequently, an isomer of 17βE2S, 17α-estradiol hemisuccinate (17αE2S), was synthesized and purified. Thirty-six mPOA-S neurons from normal cycling female rats were tested with both 17βE2S and 17αE2S. Twelve of these units responded with inhibition to 17βE2S, while none responded to 17αE2S. Furthermore, fifty extrahypothalamic (cortical, hippocampal, thalamic) neurons were tested with 17βE2S. The majority (N = 45) showed no response, three showed excitation and two inhibition to the microelectrophoresed steroid ester. These findings suggest that a specific receptor mechanism is responsible for the changes in mPOA-S unit activity, and that these effects may be important in the regulation of reproductive events.

AB - The purpose of this study was to determine the specificity of the response of medial preoptic-septal neurons (mPOA-S) to microelectrophoresed 17β-estradiol hemisuccinate (17βE2S). In vitro studies were conducted initially to determine the release of the labeled 17βE2S from multibarrel glass micropipettes. Subsequently, an isomer of 17βE2S, 17α-estradiol hemisuccinate (17αE2S), was synthesized and purified. Thirty-six mPOA-S neurons from normal cycling female rats were tested with both 17βE2S and 17αE2S. Twelve of these units responded with inhibition to 17βE2S, while none responded to 17αE2S. Furthermore, fifty extrahypothalamic (cortical, hippocampal, thalamic) neurons were tested with 17βE2S. The majority (N = 45) showed no response, three showed excitation and two inhibition to the microelectrophoresed steroid ester. These findings suggest that a specific receptor mechanism is responsible for the changes in mPOA-S unit activity, and that these effects may be important in the regulation of reproductive events.

KW - Estradiol

KW - Extrahypothalamic neurons

KW - Preoptic area

KW - Stereospecificity

KW - Transport number

UR - http://www.scopus.com/inward/record.url?scp=0017743662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017743662&partnerID=8YFLogxK

U2 - 10.1007/BF00237857

DO - 10.1007/BF00237857

M3 - Article

C2 - 563340

AN - SCOPUS:0017743662

VL - 30

SP - 43

EP - 52

JO - Experimental Brain Research

JF - Experimental Brain Research

SN - 0014-4819

IS - 1

ER -