The specific binding of ricin and its polypeptide chains to rat liver ribosomes and ribosomal subunits

Mary L. Hedblom, Daniel Cawley, L. L. Houston

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Ricin from Ricinus communis was isolated and the binding of 3H-reductively alkylated or 125I-iodinated ricin was studied by incubating the toxic protein with ribosomes and isolating the ricin-ribosome complex by centrifugation. Neither of the labeled ricin derivatives nor 3H-labeled A chain bound Escherichia coli ribosomes, but both bound rat liver ribosomes in a reproducible manner. 3H-labeled ricin bound in a ratio of 1 mol/mol of ribosomes with a dissociation constant of 3 μm as calculated from a Scatchard plot. Similarly, 3H-labeled B chain isolated from ricin also bound in a one-to-one complex with a dissociation constant of 1 μm. The binding of ricin and ricin B chain was sensitive to lactose, while the binding of reduced ricin or ricin A chain was not prevented by lactose. Reduced 125I-labeled ricin in the presence of lactose and 3H-labeled A chain bound with a ratio of 2 mol/mol of ribosomes. It was further demonstrated that 3H-labeled ricin A chain bound only to the 60S ribosomal subunit and not to the 40S ribosomal subunit. The dissociation constant for the binding was 2 μm both in the presence and absence of lactose and 2 mol of A chain were bound per mole of 60S ribosomal subunit.

Original languageEnglish (US)
Pages (from-to)46-55
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume177
Issue number1
DOIs
StatePublished - 1976
Externally publishedYes

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Ricin
Ribosome Subunits
Liver
Rats
Peptides
Ribosomes
Lactose
Eukaryotic Large Ribosome Subunits
Eukaryotic Small Ribosome Subunits
Ricinus
Centrifugation
Poisons
Escherichia coli

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The specific binding of ricin and its polypeptide chains to rat liver ribosomes and ribosomal subunits. / Hedblom, Mary L.; Cawley, Daniel; Houston, L. L.

In: Archives of Biochemistry and Biophysics, Vol. 177, No. 1, 1976, p. 46-55.

Research output: Contribution to journalArticle

Hedblom, Mary L. ; Cawley, Daniel ; Houston, L. L. / The specific binding of ricin and its polypeptide chains to rat liver ribosomes and ribosomal subunits. In: Archives of Biochemistry and Biophysics. 1976 ; Vol. 177, No. 1. pp. 46-55.
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