The somatostatin receptors SSTR1 and SSTR2 are coupled to inhibition of adenylyl cyclase in Chinese hamster ovary cells via pertussis toxin-sensitive pathways

Ray E. Hershberger, Brenda L. Newman, Tullio Florio, Jim Bunzow, Olivier Civelli, Xiao Jiang Li, Michael Forte, Philip Stork

Research output: Contribution to journalArticle

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Abstract

Somatostatin exerts multiple effects throughout the body by binding to specific somatostatin receptors. Two classes of somatostatin receptors, SRIF1 and SRIF2, have been distinguished biochemically and pharmacologically. Two cDNAs have been recently isolated that encode somatostatin receptors 1 and 2 (SSTR1 and SSTR2, respectively). The pharmacological characteristics of receptors expressing these cDNAs resemble those of the SRIF2 and SRIF1 classes of somatostatin receptors, respectively. We stably expressed the rat homologs of both receptors in Chinese hamster ovary (CHO) cells (type K1). These transfected cell lines recognized the endogenous ligands SS14 and SS28 with high affinity, whereas the synthetic analog MK678 identified only SSTR2. In preparations of CHO-SSTR1 or CHO-SSTR2 cells, SS14 and SS28 inhibited forskolin-stimulated adenylyl cyclase activity by approximately 35%, with ED50 values in the nanomolar range. The adenylyl cyclase inhibition was dependent upon the guanine nucleotide GTP and could be ablated with pertussis toxin preincubation. The present data indicate that SSTR1 and SSTR2 are coupled to inhibition of adenylyl cyclase via pertussis toxin-sensitive G- proteins.

Original languageEnglish (US)
Pages (from-to)1277-1285
Number of pages9
JournalEndocrinology
Volume134
Issue number3
DOIs
StatePublished - Mar 1994

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Somatostatin Receptors
Pertussis Toxin
Cricetulus
Adenylyl Cyclases
Ovary
Complementary DNA
Guanine Nucleotides
Colforsin
Guanosine Triphosphate
Somatostatin
GTP-Binding Proteins
Pharmacology
Ligands
Cell Line
somatostatin receptor type 1

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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The somatostatin receptors SSTR1 and SSTR2 are coupled to inhibition of adenylyl cyclase in Chinese hamster ovary cells via pertussis toxin-sensitive pathways. / Hershberger, Ray E.; Newman, Brenda L.; Florio, Tullio; Bunzow, Jim; Civelli, Olivier; Li, Xiao Jiang; Forte, Michael; Stork, Philip.

In: Endocrinology, Vol. 134, No. 3, 03.1994, p. 1277-1285.

Research output: Contribution to journalArticle

Hershberger, Ray E. ; Newman, Brenda L. ; Florio, Tullio ; Bunzow, Jim ; Civelli, Olivier ; Li, Xiao Jiang ; Forte, Michael ; Stork, Philip. / The somatostatin receptors SSTR1 and SSTR2 are coupled to inhibition of adenylyl cyclase in Chinese hamster ovary cells via pertussis toxin-sensitive pathways. In: Endocrinology. 1994 ; Vol. 134, No. 3. pp. 1277-1285.
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