The SK2-long isoform directs synaptic localization and function of SK2-containing channels

Duane Allen, Chris T. Bond, Rafael Lujàn, Carmen Ballesteros-Merino, Mike T. Lin, Kang Wang, Nathan Klett, Masahiko Watanabe, Ryuichi Shigemoto, Robert W. Stackman Jr, James Maylie, John Adelman

Research output: Contribution to journalArticle

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Abstract

SK2-containing channels are expressed in the postsynaptic density (PSD) of dendritic spines on mouse hippocampal area CA1 pyramidal neurons and influence synaptic responses, plasticity and learning. The Sk2 gene (also known as Kcnn2) encodes two isoforms that differ only in the length of their N-terminal domains. SK2-long (SK2-L) and SK2-short (SK2-S) are coexpressed in CA1 pyramidal neurons and likely form heteromeric channels. In mice lacking SK2-L (SK2-S only mice), SK2-S-containing channels were expressed in the extrasynaptic membrane, but were excluded from the PSD. The SK channel contribution to excitatory postsynaptic potentials was absent in SK2-S only mice and was restored by SK2-L re-expression. Blocking SK channels increased the amount of long-term potentiation induced in area CA1 in slices from wild-type mice but had no effect in slices from SK2-S only mice. Furthermore, SK2-S only mice outperformed wild-type mice in the novel object recognition task. These results indicate that SK2-L directs synaptic SK2-containing channel expression and is important for normal synaptic signaling, plasticity and learning.

Original languageEnglish (US)
Pages (from-to)744-749
Number of pages6
JournalNature Neuroscience
Volume14
Issue number6
DOIs
StatePublished - Jun 2011

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Protein Isoforms
Post-Synaptic Density
Neuronal Plasticity
Pyramidal Cells
Learning
Dendritic Spines
Long-Term Potentiation
Excitatory Postsynaptic Potentials
Membranes
Genes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Allen, D., Bond, C. T., Lujàn, R., Ballesteros-Merino, C., Lin, M. T., Wang, K., ... Adelman, J. (2011). The SK2-long isoform directs synaptic localization and function of SK2-containing channels. Nature Neuroscience, 14(6), 744-749. https://doi.org/10.1038/nn.2832

The SK2-long isoform directs synaptic localization and function of SK2-containing channels. / Allen, Duane; Bond, Chris T.; Lujàn, Rafael; Ballesteros-Merino, Carmen; Lin, Mike T.; Wang, Kang; Klett, Nathan; Watanabe, Masahiko; Shigemoto, Ryuichi; Stackman Jr, Robert W.; Maylie, James; Adelman, John.

In: Nature Neuroscience, Vol. 14, No. 6, 06.2011, p. 744-749.

Research output: Contribution to journalArticle

Allen, D, Bond, CT, Lujàn, R, Ballesteros-Merino, C, Lin, MT, Wang, K, Klett, N, Watanabe, M, Shigemoto, R, Stackman Jr, RW, Maylie, J & Adelman, J 2011, 'The SK2-long isoform directs synaptic localization and function of SK2-containing channels', Nature Neuroscience, vol. 14, no. 6, pp. 744-749. https://doi.org/10.1038/nn.2832
Allen D, Bond CT, Lujàn R, Ballesteros-Merino C, Lin MT, Wang K et al. The SK2-long isoform directs synaptic localization and function of SK2-containing channels. Nature Neuroscience. 2011 Jun;14(6):744-749. https://doi.org/10.1038/nn.2832
Allen, Duane ; Bond, Chris T. ; Lujàn, Rafael ; Ballesteros-Merino, Carmen ; Lin, Mike T. ; Wang, Kang ; Klett, Nathan ; Watanabe, Masahiko ; Shigemoto, Ryuichi ; Stackman Jr, Robert W. ; Maylie, James ; Adelman, John. / The SK2-long isoform directs synaptic localization and function of SK2-containing channels. In: Nature Neuroscience. 2011 ; Vol. 14, No. 6. pp. 744-749.
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