The SHI-3 iron transport island of Shigella boydii 0-1392 carries the genes for aerobactin synthesis and transport

Georgiana Purdy, S. M. Payne

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In Shigella boydii 0-1392, genes encoding the synthesis and transport of the hydroxamate siderophore aerobactin are located within a 21-kb iron transport island between lysU and the pheU tRNA gene. DNA sequence analysis of the S. boydii 0-1392 island, designated SHI-3 for Shigella island 3, revealed a conserved aerobactin operon associated with a P4 prophage-like integrase gene and numerous insertion sequences (IS). SHI-3 is present at the pheU tRNA locus in some S. boydii isolates but not in others. The map locations of the aerobactin genes vary among closely related species. The association of the aerobactin operon with phage genes and mobile elements and its presence at different locations within the genomes of enteric pathogens suggest that these virulence-enhancing genes may have been acquired by bacteriophage integration or IS element-mediated transposition. An S. boydii aerobactin synthesis mutant, 0-1392 iucB, was constructed and was similar to the wild type in tissue culture assays of invasion and intercellular spread.

Original languageEnglish (US)
Pages (from-to)4176-4182
Number of pages7
JournalJournal of Bacteriology
Volume183
Issue number14
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Shigella boydii
Islands
Iron
Genes
Operon
Transfer RNA
Bacteriophages
Prophages
Integrases
Siderophores
DNA Transposable Elements
Shigella
Insertional Mutagenesis
DNA Sequence Analysis
Virulence
aerobactin
Genome

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The SHI-3 iron transport island of Shigella boydii 0-1392 carries the genes for aerobactin synthesis and transport. / Purdy, Georgiana; Payne, S. M.

In: Journal of Bacteriology, Vol. 183, No. 14, 2001, p. 4176-4182.

Research output: Contribution to journalArticle

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