The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor pu.1

Ralph Paul, Scott Schuetze, Susan L. Kozak, Christine A. Kozak, David Kabat

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 inRNA were isolated and setpeneed. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained common differences from that sequence, including in the coding region four extra nucleotides that altered the reading frame. The properly translated protein is identical to Pu.l, a transcription activation factor that is related to the ets oncogene family. Genetic methods were used to map Sfpi-1 with respect, to other loci on mouse chromosome 2. Our results suggest that Pu.1 blocks erythroblast differentiation and thereby causes immortalization.

Original languageEnglish (US)
Pages (from-to)464-467
Number of pages4
JournalJournal of virology
Volume65
Issue number1
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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