Abstract
Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 inRNA were isolated and setpeneed. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained common differences from that sequence, including in the coding region four extra nucleotides that altered the reading frame. The properly translated protein is identical to Pu.l, a transcription activation factor that is related to the ets oncogene family. Genetic methods were used to map Sfpi-1 with respect, to other loci on mouse chromosome 2. Our results suggest that Pu.1 blocks erythroblast differentiation and thereby causes immortalization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 464-467 |
| Number of pages | 4 |
| Journal | Journal of Virology |
| Volume | 65 |
| Issue number | 1 |
| State | Published - 1991 |
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ASJC Scopus subject areas
- Immunology
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The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor pu.1. / Paul, Ralph; Schuetze, Scott; Kozak, Susan L.; Kozak, Christine A.; Kabat, David.
In: Journal of Virology, Vol. 65, No. 1, 1991, p. 464-467.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor pu.1
AU - Paul, Ralph
AU - Schuetze, Scott
AU - Kozak, Susan L.
AU - Kozak, Christine A.
AU - Kabat, David
PY - 1991
Y1 - 1991
N2 - Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 inRNA were isolated and setpeneed. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained common differences from that sequence, including in the coding region four extra nucleotides that altered the reading frame. The properly translated protein is identical to Pu.l, a transcription activation factor that is related to the ets oncogene family. Genetic methods were used to map Sfpi-1 with respect, to other loci on mouse chromosome 2. Our results suggest that Pu.1 blocks erythroblast differentiation and thereby causes immortalization.
AB - Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 inRNA were isolated and setpeneed. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained common differences from that sequence, including in the coding region four extra nucleotides that altered the reading frame. The properly translated protein is identical to Pu.l, a transcription activation factor that is related to the ets oncogene family. Genetic methods were used to map Sfpi-1 with respect, to other loci on mouse chromosome 2. Our results suggest that Pu.1 blocks erythroblast differentiation and thereby causes immortalization.
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M3 - Article
C2 - 1985210
AN - SCOPUS:0025983405
VL - 65
SP - 464
EP - 467
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 1
ER -