The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor pu.1

Ralph Paul, Scott Schuetze, Susan L. Kozak, Christine A. Kozak, David Kabat

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 inRNA were isolated and setpeneed. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained common differences from that sequence, including in the coding region four extra nucleotides that altered the reading frame. The properly translated protein is identical to Pu.l, a transcription activation factor that is related to the ets oncogene family. Genetic methods were used to map Sfpi-1 with respect, to other loci on mouse chromosome 2. Our results suggest that Pu.1 blocks erythroblast differentiation and thereby causes immortalization.

Original languageEnglish (US)
Pages (from-to)464-467
Number of pages4
JournalJournal of Virology
Volume65
Issue number1
StatePublished - 1991

Fingerprint

Proto-Oncogene Proteins c-ets
erythroblasts
Leukemia, Erythroblastic, Acute
oncogenes
transcriptional activation
transcription factors
Complementary DNA
researchers
nucleotides
chromosomes
Reading Frames
Erythroblasts
loci
Chromosomes, Human, Pair 2
mice
Oncogenes
Transcriptional Activation
Transcription Factors
Nucleotides
proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Paul, R., Schuetze, S., Kozak, S. L., Kozak, C. A., & Kabat, D. (1991). The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor pu.1. Journal of Virology, 65(1), 464-467.

The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor pu.1. / Paul, Ralph; Schuetze, Scott; Kozak, Susan L.; Kozak, Christine A.; Kabat, David.

In: Journal of Virology, Vol. 65, No. 1, 1991, p. 464-467.

Research output: Contribution to journalArticle

Paul, R, Schuetze, S, Kozak, SL, Kozak, CA & Kabat, D 1991, 'The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor pu.1', Journal of Virology, vol. 65, no. 1, pp. 464-467.
Paul, Ralph ; Schuetze, Scott ; Kozak, Susan L. ; Kozak, Christine A. ; Kabat, David. / The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor pu.1. In: Journal of Virology. 1991 ; Vol. 65, No. 1. pp. 464-467.
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