The sequence of human serum albumin cDNA and its expression in E. coli

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Abstract

A recombinant plasmid has been constructed which contains the mature protein coding region of the human serum albumin (HSA) gene. Bacteria containing this plasmid synthesize HSA protein under control of the E. coli trp promoter-operator. The DNA sequence and predicted protein sequence of HSA were determined from the cDNA plasmid and are compared to existing data obtained from direct protein sequencing. The DNA sequence predicts a mature protein of 585 amino acids preceded by a 24 amino acid "prepro' peptide.

Original languageEnglish (US)
Pages (from-to)6103-6114
Number of pages12
JournalNucleic Acids Research
Volume9
Issue number22
DOIs
Publication statusPublished - Nov 25 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Lawn, R. M., Adelman, J., Bock, S. C., Franke, A. E., Houck, C. M., Najarian, R., ... Wion, K. L. (1981). The sequence of human serum albumin cDNA and its expression in E. coli. Nucleic Acids Research, 9(22), 6103-6114. https://doi.org/10.1093/nar/9.22.6103