The role of the suckling stimulus in regulating pituitary prolactin mRNA in the rat

Ling Ru Lee, Daniel J. Haisenleder, John C. Marshall, M. Susan Smith

    Research output: Contribution to journalArticle

    20 Scopus citations

    Abstract

    Prolactin (PRL) gene expression and the synthesis and secretion of PRL were examined in ovarian-intact lactating rats suckling eight pups on 10 days postpartum. Plasma samples were assayed for PRL concentrations, and pituitary glands were analyzed for total PRL content and PRL mRNA levels. We found that suckling-induced hyperprolactinemia was associated with very high levels of plasma PRL and a doubling in pituitary PRL mRNA levels, whereas pituitary PRL content was not changed. Removal of the suckling pups decreased plasma PRL concentrations 15-fold within 24 h. This decrease in PRL secretion was not accompanied by any significant change in pituitary PRL content. Evidently, both synthesis and secretion of PRL were decreased in the pituitary gland within 24 h following cessation of suckling, as pituitary PRL mRNA content had returned to diestrous levels at this time. To determine whether or not ovarian steroids might have contributed to the changes in PRL synthesis and secretion during lactation and after withdrawal of the suckling stimulus, the experiments were repeated in lactating rats ovariectomized (OVX) on day 2 postpartum. The results in these OVX rats were qualitatively similar to those described in ovarian-intact rats. We concluded from these findings that the stimulus of suckling induces increases in PRL mRNA levels in the pituitary which provides for the increased PRL synthesis accompanying increased PRL secretion. The cessation of suckling led to prompt decreases in PRL synthesis and secretion within 24 h.

    Original languageEnglish (US)
    Pages (from-to)243-249
    Number of pages7
    JournalMolecular and Cellular Endocrinology
    Volume64
    Issue number2
    DOIs
    StatePublished - Jul 1989

    Keywords

    • Lactation
    • Pituitary prolactin
    • Plasma prolactin
    • Prolactin mRNA

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Endocrinology

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